PXD010125

PXD010125 is an original dataset announced via ProteomeXchange.

Title	Mouse epididymis LC-MSMS - Male Fertility Potential Molecular Mechanisms Revealed by iTRAQ-Based Quantitative Proteomic Analysis of the Epididymis from Wip1-/- Mice
Description	The wild-type p53-induced phosphatase 1 (WIP1) frustrated mice exhibited defects in reproductive organs. This study aimed to understand how Wip1 deficiency affects the spermatogenesis or maturation. We employed the Wip1-/- mouse model and conducted a gel-free iTRAQ LC-MS/MS based quantitative proteomics analysis of whole epididymis including the sperms and somatic tissue. A total of 8763 proteins were identified, of which 91 were significantly differentially expressed proteins (DEPs) in the Wip1 depleted mice. Four DEPs (PRM2, ODF1, PIWIL1 and KLHL10) were confirmed with western blotting. The DEPs enriched in biological process of reproduction were identified by GO analysis and further confirmed in the mouse phenotype database. Pathway analysis suggested that Smac/Diablo-mediated apoptosis pathway and SERPINA3-mediated inflammatory process might contribute to the atrophy and the marked sperm decrease in epididymis. Network analysis of productivity related DEPs revealed possible interactions that WIP1 might affect the sperm maturation by decreasing the outer dense fiber protein 1 (ODF1) and protamine-2 (PRM2), and increasing the PIWIL1/MIWI through p53. PRM2 was down-regulated and PIWIL1 was up-regulated by immunohistochemistry staining. It was further confirmed that the spermatid deficiency began from the testis observed by HE staining. Therefore, WIP1 disruption caused the deficiency of spermatogenesis potentially through regulating the expression of the above DEPs and pathways.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:45:46.056.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	pengxia niu
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iTRAQ8plex-116 reporter+balance reagent acylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-06-14 01:55:55	ID requested
1	2019-01-16 00:52:55	announced
⏵ 2	2024-10-22 04:45:52	announced	2024-10-22: Updated project metadata.

10.1089/omi.2018.0155;

Niu P, Wei Y, Gao Q, Zhang X, Hu Y, Qiu Y, Mu Y, Li K, Mice. OMICS, 23(1):54-66(2019) [pubmed]

curator keyword: Biological

submitter keyword: LC-MSMS,Mouse, sperm, iTRAQ, epididymis

Li Kui
contact affiliation	Insititute of Animal Sciences,Chinese Academy of Agricultural Sciences
contact email	likui@caas.cn
lab head
pengxia niu
contact affiliation	Chinese Academy of Agricultural Sciences
contact email	pengxianiu@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD010125
     1. Label: PRIDE project
     2. Name: Mouse epididymis LC-MSMS - Male Fertility Potential Molecular Mechanisms Revealed by iTRAQ-Based Quantitative Proteomic Analysis of the Epididymis from Wip1-/- Mice