PXD010120

PXD010120 is an original dataset announced via ProteomeXchange.

Title	Laboratory adaptation of Bacillus anthracis
Description	The spore forming pathogen Bacillus anthracis is the etiologic agent of anthrax in humans and animals. It cycles through infected hosts as vegetative cells and is eventually introduced into the environment where it generates an endospore resistant to many harsh conditions. The endospores are subsequently ingested by the next host to begin the next cycle. Outbreaks of anthrax occur regularly worldwide in wildlife and livestock, and the potential for human infection exists whenever humans encounter infected animals. It is also possible to encounter intentional releases of anthrax spores, as was the case in October 2001. Consequently, it is important to be able to rapidly establish the provenance of infectious strains of B. anthracis. Here, we compare protein expression in seven low-passage wild isolates and four laboratory strains of B. anthracis grown under identical conditions using LC-MS/MS proteomic analysis. Of the 1,023 total identified proteins, 96 had significant abundance differences between wild and laboratory strains. Of those, 28 proteins directly related to sporulation were upregulated in wild isolates, with expression driven by Spo0A, CodY, and AbrB/ScoC. In addition, we observed evidence of changes in cell division and fatty acid biosynthesis between the two classes of strains, despite being grown under identical experimental conditions. These results suggest wild B. anthracis cells are more highly tuned to sporulate than their laboratory cousins, and this difference should be exploited as a method to differentiate between laboratory adapted cultures and low passage wild strains isolated during an anthrax outbreak. This knowledge should distinguish between intentional releases and exposure to strains in nature providing a basis for the type of response by public health officials and investigators.
HostingRepository	PRIDE
AnnounceDate	2018-12-04
AnnouncementXML	Submission_2019-01-03_02:28:30.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Owen Leiser
SpeciesList	scientific name: Bacillus anthracis str. Ames; NCBI TaxID: 198094;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2018-06-14 01:33:41	ID requested
1	2018-12-04 08:20:01	announced
⏵ 2	2019-01-03 02:28:31	announced	Updated publication reference for PubMed record(s): 30557394.

Leiser OP, Blackburn JK, Hadfield TL, Kreuzer HW, Wunschel DS, Bruckner-Lea CJ, Laboratory strains of Bacillus anthracis exhibit pervasive alteration in expression of proteins related to sporulation under laboratory conditions relative to genetically related wild strains. PLoS One, 13(12):e0209120(2018) [pubmed]

curator keyword: Biological

submitter keyword: Anthrax, Bacillus anthracis, evolution, sporulation, shotgun proteomics

Helen Kreuzer
contact affiliation	Pacific Northwest National Laboratory
contact email	Helen.Kreuzer@pnnl.gov
lab head
Owen Leiser
contact affiliation	Pacific Northwest National Laboratory
contact email	Owen.Leiser@pnnl.gov
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD010120
     1. Label: PRIDE project
     2. Name: Laboratory adaptation of Bacillus anthracis