PXD010113

PXD010113 is an original dataset announced via ProteomeXchange.

Title	limpet teeth LC-MSMS
Description	Limpets are marine mollusks using mineralized teeth, one of the hardest and strongest biomaterials, to feed on algae on intertidal rocks. However, most of studies only focuses on the ultrastructure and chemical composition of the teeth while the molecular information is largely unknown, limiting our understanding of this unique and fundamental biomineralization process. In this study, we investigated the teeth of limpet Cellana toreuma from three perspectives: 1) by using electron microscopy to observe the microstructure of the teeth; 2) by using proteomics and RNA-seq to investigate the proteins involved in the limpet teeth and 3) by in vitro crystallization experiment combined with Raman spectroscopy to investigate the effects of proteins and chitin framework on crystal formation. It is found that the limpets formed alternatively tricuspid teeth and unicuspid teeth. Small nanoneedles were densely packed at the tips or leading regions of the cusps. In contrast, big nanoneedles resembling chemical synthesized goethite were loosely packed in the trailing regions of the cusps. Proteins extracted from the whole teeth such as ferritin, peroxiredoxin, arginine kinase, GTPase-Rabs and clathrin were identified by proteomics. Goethite-binding experiment coupled with proteomics and RNA-seq highlighted six chitin-binding proteins (CtCBPs). Furthermore, these proteins or the framework chitin only induced packing of crystals without affecting their crystal polymorphs in vitro. Taken together, the limpets formed hierarchical teeth across different length scales through preformed framework and secreted complex proteins; in addition, the chitin could also be an important player in controlling crystallinity and crystal packing in vivo. This study provides insight into the unique biomineralization process in the limpet teeth at the molecular levels, which may guide biomimetic strategies aimed at designing hard materials at room temperature.
HostingRepository	PRIDE
AnnounceDate	2019-03-26
AnnouncementXML	Submission_2019-03-26_01:53:19.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yadong Wang
SpeciesList	scientific name: Cellana toreuma; NCBI TaxID: 42758;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2018-06-13 05:55:26	ID requested
⏵ 1	2019-03-26 01:53:20	announced

Wang Y, Liu C, Du J, Huang J, Zhang S, Zhang R, The Microstructure, Proteomics and Crystallization of the Limpet Teeth. Proteomics, 18(19):e1800194(2018) [pubmed]

curator keyword: Biological

submitter keyword: limpet teeth,Cellana toreuma,

Rongqing Zhang
contact affiliation	school of life sciences,Tsinghua University,Beijing 100084,China
contact email	rqzhang@mail.tsinghua.edu.cn
lab head
Yadong Wang
contact affiliation	Tsinghua university
contact email	wangyadong_2009@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/03/PXD010113

PRIDE project URI

• PRIDE
  1. PXD010113
     1. Label: PRIDE project
     2. Name: limpet teeth LC-MSMS