PXD010111

PXD010111 is an original dataset announced via ProteomeXchange.

Title	Crosslinking MS to elucidate the Architecture of the Nucleosome Remodelling and Deacetylase (NuRD) complex
Description	The Nucleosome Remodeling and Deacetylase (NuRD) complex is essential for development in complex animals but has been refractory to detailed analysis because of its low abundance and resistance to recombinant production. In combination with other techniques, crosslinking mass spectrometry was used to elucidate the structure of the Nucleosome Remodelling and Deacetylase (NuRD) complex. Natively-purified NuRD, and four recombinantly-expressed NuRD subcomplexes, and a PWWP2A-MTA-HDAC-RBBP alternative ‘NuRD-like’ complex were subjected to crosslinking with DSS, ADH, BS3 and DMTMM.
HostingRepository	PRIDE
AnnounceDate	2021-01-08
AnnouncementXML	Submission_2021-01-08_00:05:22.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jason Low
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive; Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2018-06-13 05:40:17	ID requested
⏵ 1	2021-01-08 00:05:23	announced

Low JKK, Silva APG, Sharifi Tabar M, Torrado M, Webb SR, Parker BL, Sana M, Smits C, Schmidberger JW, Brillault L, Jackman MJ, Williams DC, Blobel GA, Hake SB, Shepherd NE, Landsberg MJ, Mackay JP, The Nucleosome Remodeling and Deacetylase Complex Has an Asymmetric, Dynamic, and Modular Architecture. Cell Rep, 33(9):108450(2020) [pubmed]

submitter keyword: NuRD, crosslinking MS, structural proteomics

Joel Mackay
contact affiliation	School of Life and Environmental Sciences, University of Sydney, NSW Australia
contact email	joel.mackay@sydney.edu.au
lab head
Jason Low
contact affiliation	The University of Sydney
contact email	jason.low@sydney.edu.au
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD010111
     1. Label: PRIDE project
     2. Name: Crosslinking MS to elucidate the Architecture of the Nucleosome Remodelling and Deacetylase (NuRD) complex