PXD010102 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Sample preparation procedure for mass spectrometric analysis of plant histones |
Description | The protocol for preparing samples of plant histones prior to LC-MS/MS analysis was established, using a filter-aided sample preparation (FASP) technique to remove natural contaminants and chemical additives incompatible with MS-based procedures. The protocol consists of nuclei isolation, histone extraction into acidic solvent, protein propionylation and on-membrane digestion, then peptide propionylation in solution. Two variants of the derivatization protocol, in-solution propionylation in the vial (PROP-in-SOL) and propionylation on the filter unit (PROP-on-FILTER), were compared by efficiency of the labeling and by abundance of identified histone peptide forms. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:47:40.500.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Gabriela Lochmanova |
SpeciesList | scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702; |
ModificationList | monomethylated residue; phosphorylated residue; N6-propanoyl-L-lysine; acetylated residue; N6,N6-dimethyl-L-lysine; N6,N6,N6-trimethyl-L-lysine |
Instrument | LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-06-12 02:32:54 | ID requested | |
1 | 2018-09-20 04:55:09 | announced | |
2 | 2018-09-21 00:30:21 | announced | Updated project metadata. |
3 | 2018-10-09 08:02:45 | announced | Updated project metadata. |
⏵ 4 | 2024-10-22 04:47:42 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.3389/fpls.2018.01373; |
Ledvinov, á D, Mikul, á, š, ek K, Kucha, ř, í, kov, á H, Brabencov, á S, Fojtov, á M, Zdr, á, hal Z, Lochmanov, á G, Filter-Aided Sample Preparation Procedure for Mass Spectrometric Analysis of Plant Histones. Front Plant Sci, 9():1373(2018) [pubmed] |
Keyword List
curator keyword: Technical |
submitter keyword: Arabidopsis thaliana, Mass spectrometry, Post-translational modifications, Epigenetics,Histone derivatization, Filter-aided sample preparation |
Contact List
Zbynek Zdrahal |
contact affiliation | CEITEC-MU, Masaryk University, Brno, Czech Republic |
contact email | zdrahal@sci.muni.cz |
lab head | |
Gabriela Lochmanova |
contact affiliation | CEITEC-MU, Masaryk University, Brno |
contact email | g.lochmanova@seznam.cz |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD010102
- Label: PRIDE project
- Name: Sample preparation procedure for mass spectrometric analysis of plant histones