⮝ Full datasets listing

PXD010102

PXD010102 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSample preparation procedure for mass spectrometric analysis of plant histones
DescriptionThe protocol for preparing samples of plant histones prior to LC-MS/MS analysis was established, using a filter-aided sample preparation (FASP) technique to remove natural contaminants and chemical additives incompatible with MS-based procedures. The protocol consists of nuclei isolation, histone extraction into acidic solvent, protein propionylation and on-membrane digestion, then peptide propionylation in solution. Two variants of the derivatization protocol, in-solution propionylation in the vial (PROP-in-SOL) and propionylation on the filter unit (PROP-on-FILTER), were compared by efficiency of the labeling and by abundance of identified histone peptide forms.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:47:40.500.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterGabriela Lochmanova
SpeciesList scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationListmonomethylated residue; phosphorylated residue; N6-propanoyl-L-lysine; acetylated residue; N6,N6-dimethyl-L-lysine; N6,N6,N6-trimethyl-L-lysine
InstrumentLTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-06-12 02:32:54ID requested
12018-09-20 04:55:09announced
22018-09-21 00:30:21announcedUpdated project metadata.
32018-10-09 08:02:45announcedUpdated project metadata.
42024-10-22 04:47:42announced2024-10-22: Updated project metadata.
Publication List
10.3389/fpls.2018.01373;
Ledvinov, á D, Mikul, á, š, ek K, Kucha, ř, í, kov, á H, Brabencov, á S, Fojtov, á M, Zdr, á, hal Z, Lochmanov, á G, Filter-Aided Sample Preparation Procedure for Mass Spectrometric Analysis of Plant Histones. Front Plant Sci, 9():1373(2018) [pubmed]
Keyword List
curator keyword: Technical
submitter keyword: Arabidopsis thaliana, Mass spectrometry, Post-translational modifications, Epigenetics,Histone derivatization, Filter-aided sample preparation
Contact List
Zbynek Zdrahal
contact affiliationCEITEC-MU, Masaryk University, Brno, Czech Republic
contact emailzdrahal@sci.muni.cz
lab head
Gabriela Lochmanova
contact affiliationCEITEC-MU, Masaryk University, Brno
contact emailg.lochmanova@seznam.cz
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/09/PXD010102
PRIDE project URI
Repository Record List
[ + ]