PXD010102

PXD010102 is an original dataset announced via ProteomeXchange.

Title	Sample preparation procedure for mass spectrometric analysis of plant histones
Description	The protocol for preparing samples of plant histones prior to LC-MS/MS analysis was established, using a filter-aided sample preparation (FASP) technique to remove natural contaminants and chemical additives incompatible with MS-based procedures. The protocol consists of nuclei isolation, histone extraction into acidic solvent, protein propionylation and on-membrane digestion, then peptide propionylation in solution. Two variants of the derivatization protocol, in-solution propionylation in the vial (PROP-in-SOL) and propionylation on the filter unit (PROP-on-FILTER), were compared by efficiency of the labeling and by abundance of identified histone peptide forms.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:47:40.500.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gabriela Lochmanova
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	monomethylated residue; phosphorylated residue; N6-propanoyl-L-lysine; acetylated residue; N6,N6-dimethyl-L-lysine; N6,N6,N6-trimethyl-L-lysine
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2018-06-12 02:32:54	ID requested
1	2018-09-20 04:55:09	announced
2	2018-09-21 00:30:21	announced	Updated project metadata.
3	2018-10-09 08:02:45	announced	Updated project metadata.
⏵ 4	2024-10-22 04:47:42	announced	2024-10-22: Updated project metadata.

10.3389/fpls.2018.01373;

Ledvinov, á D, Mikul, á, š, ek K, Kucha, ř, í, kov, á H, Brabencov, á S, Fojtov, á M, Zdr, á, hal Z, Lochmanov, á G, Filter-Aided Sample Preparation Procedure for Mass Spectrometric Analysis of Plant Histones. Front Plant Sci, 9():1373(2018) [pubmed]

curator keyword: Technical

submitter keyword: Arabidopsis thaliana, Mass spectrometry, Post-translational modifications, Epigenetics,Histone derivatization, Filter-aided sample preparation

Zbynek Zdrahal
contact affiliation	CEITEC-MU, Masaryk University, Brno, Czech Republic
contact email	zdrahal@sci.muni.cz
lab head
Gabriela Lochmanova
contact affiliation	CEITEC-MU, Masaryk University, Brno
contact email	g.lochmanova@seznam.cz
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/09/PXD010102

PRIDE project URI

• PRIDE
  1. PXD010102
     1. Label: PRIDE project
     2. Name: Sample preparation procedure for mass spectrometric analysis of plant histones