PXD010101

PXD010101 is an original dataset announced via ProteomeXchange.

Title	Stress-induced MazF-mediated proteins in Escherichia coli
Description	Escherichia coli (E. coli) mazEF is an extensively studied stress-induced toxin-antitoxin (TA) system. The toxin MazF is an endoribonuclease that cleaves RNAs at ACA sites. Thereby, under stress the induced MazF generates a Stress induced Translation Machinery (STM), composed of MazF processed mRNAs and selective ribosomes that specifically translate the processed mRNAs. Here we performed a proteomic analysis of all the E. coli stress-induced proteins that are mediated through chromosomally borne mazF gene . We show that the mRNAs of almost all of them is characterized by the presence of an ACA site up to 100 nucleotides upstream to the AUG initiator. Thereby, under stressful conditions the induced MazF is processing mRNAs that are translated by STM. Furthermore, the presence of the ACA site far enough upstream (up to 100 nucleotides) to the AUG initiator may still permit translation by the canonical translation machinery. Thus, such dual translation mechanisms, enable under stress also to prepare proteins for immediate functions while coming back to normal growth conditions.
HostingRepository	PRIDE
AnnounceDate	2019-02-25
AnnouncementXML	Submission_2019-02-25_00:47:58.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tamar Ziv
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	monohydroxylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-06-12 02:26:56	ID requested
⏵ 1	2019-02-25 00:47:59	announced

Dataset with its publication pending

curator keyword: Biological

submitter keyword: MazF stress silac

Hanna Engelberg-Kulka
contact affiliation	1Department of Microbiology and Molecular Genetics, IMRIC, The Hebrew University- Hadassah Medical School, Jerusalem 91120, Israel
contact email	hannae@ekmd.huji.ac.il
lab head
Tamar Ziv
contact affiliation	Technion
contact email	tamarz@tx.technion.ac.il
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD010101
     1. Label: PRIDE project
     2. Name: Stress-induced MazF-mediated proteins in Escherichia coli