PXD010100

PXD010100 is an original dataset announced via ProteomeXchange.

Title	LC-MS/MS secretome analysis of Pleurotus ostreatus PC9 (wild type) and two transformants
Description	The white rot fungi Pleurotus ostreatus was studied for its potential as a biological pretreatment agent, i.e- its potential for decomposing lignocellulosic biomass. P. ostreatus PC9 was manipulate to either overexpress or eliminate (by gene replacement) the transcriptional regulator CRE1, known to act as a repressor in the process of carbon catabolite repression. The parental PC9 and the two transformants were grown on microcrystalline cellulose and wheat straw (3 replicates each) and the content of the resulted secretomes was analyzed by LC-MS/MS. An extensive range of carbohydrate active enzymes (CAZymes) were affected by the modification of cre1 expression levels. The three fungi revealed also differentiation towards the carbon source used in the growth media.
HostingRepository	PRIDE
AnnounceDate	2018-08-06
AnnouncementXML	Submission_2018-08-06_04:24:35.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shahar Yoav
SpeciesList	scientific name: Pleurotus ostreatus PC9; NCBI TaxID: 1137139;
ModificationList	monohydroxylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-06-12 02:18:06	ID requested
⏵ 1	2018-08-06 04:24:35	announced

Dataset with its publication pending

curator keyword: Biological

submitter keyword: Pleurotus ostreatus, secretome, plant cell wall degrading enzymes, biological pretreatment

Prof. Yitzhak Hadar
contact affiliation	Department of Plant Pathology and Microbiology, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, 76100, Israel
contact email	yitzhak.hadar@mail.huji.ac.il
lab head
Shahar Yoav
contact affiliation	PhD student
contact email	Shaharyoav@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD010100

PRIDE project URI

• PRIDE
  1. PXD010100
     1. Label: PRIDE project
     2. Name: LC-MS/MS secretome analysis of Pleurotus ostreatus PC9 (wild type) and two transformants