PXD010097

PXD010097 is an original dataset announced via ProteomeXchange.

Title	EIF3F regulates cell migration and metastasis in human lung adenocarcinoma
Description	The basic understanding of the biological effects of eukaryotic translation initiation factors (EIFs) remains incomplete. Here, we analyzed the function of EIF3F, which is commonly found to be overexpressed in human lung adenocarcinoma, and discovered that EIF3F promotes cell migration and metastasis in vivo. The underpinning molecular mechanisms involved the upregulation of a cluster of 34 metastasis-promoting genes including Snail2 (SLUG), as revealed by proteomics combined with immuno-affinity purification of EIF3F and ChIP-seq/Q-PCR analyses. The interaction between EIF3F and Signal Transducer and Activator of Transcription 3 (STAT3) controlled the EIF3F-mediated increase in Snail2 expression and cellular invasion, which were specifically abrogated using the STAT3 inhibitor nifuroxazide. Our findings demonstrate the role of EIF3F in the molecular control of cell migration, invasion and metastasis.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:45:36.205.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dupuy Jean-William
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	deaminated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-06-12 02:05:41	ID requested
1	2019-09-25 07:54:57	announced
⏵ 2	2024-10-22 04:45:38	announced	2024-10-22: Updated project metadata.

Esteves P, Dard L, Brillac A, Hubert C, Sarlak S, Rousseau B, Dumon E, Izotte J, Bonneu M, Lacombe D, Dupuy JW, Amoedo N, Rossignol R, Nuclear control of lung cancer cells migration, invasion and bioenergetics by eukaryotic translation initiation factor 3F. Oncogene, 39(3):617-636(2020) [pubmed]

10.1038/s41388-019-1009-x;

curator keyword: Biological

submitter keyword: cancer,Lung, human lung adenocarcinoma, label-free quantitative proteome analysis

Rodrigue Rossignol
contact affiliation	INSERM U1211, University of Bordeaux, CHU Pellegrin, Ecole des Sages Femmes, Place Amélie Raba Léon, 33000 Bordeaux
contact email	rodrigue.rossignol@u-bordeaux.fr
lab head
Dupuy Jean-William
contact affiliation	Plateforme Proteome Bordeaux
contact email	jean-william.dupuy@u-bordeaux.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/09/PXD010097

PRIDE project URI

• PRIDE
  1. PXD010097
     1. Label: PRIDE project
     2. Name: EIF3F regulates cell migration and metastasis in human lung adenocarcinoma