PXD010092

PXD010092 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of extracellular matrix remodeling during zebrafish heart regeneration
Description	Adult zebrafish, in contrast to mammals, are able to regenerate their hearts in response to injury or experimental amputation. Our understanding of the cellular and molecular bases that underlie this process, although fragmentary, has increased significantly over the last years. However, the role of the extracellular matrix (ECM) during zebrafish heart regeneration has been comparatively rarely explored. Here, we set out to characterize the ECM protein composition in adult zebrafish hearts, and whether it changed during the regenerative response. For this purpose, we first established a decellularization protocol of adult zebrafish ventricles that significantly enriched the yield of ECM proteins. We then performed proteomic analyses of decellularized control hearts and at different times of regeneration. Our results show a dynamic change in ECM protein composition, most evident at the earliest (7 days post-amputation) time-point analyzed. Regeneration associated with sharp increases in specific ECM proteins, and with an overall decrease in collagens and cytoskeletal proteins. We finally tested by atomic force microscopy that the changes in ECM composition translated to decreased ECM stiffness. Our cumulative results identify changes in the protein composition and mechanical properties of the zebrafish heart ECM during regeneration.
HostingRepository	PRIDE
AnnounceDate	2019-08-26
AnnouncementXML	Submission_2019-08-26_07:58:52.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Francesc Canals
SpeciesList	scientific name: Danio rerio (Zebrafish) (Brachydanio rerio); NCBI TaxID: 7955;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	maXis

Revision	Datetime	Status	ChangeLog Entry
0	2018-06-11 06:11:32	ID requested
⏵ 1	2019-08-26 07:58:53	announced

Garcia-Puig A, Mosquera JL, Jim, é, nez-Delgado S, Garc, í, a-Pastor C, Jorba I, Navajas D, Canals F, Raya A, Proteomics Analysis of Extracellular Matrix Remodeling During Zebrafish Heart Regeneration. Mol Cell Proteomics, 18(9):1745-1755(2019) [pubmed]

curator keyword: Biological

submitter keyword: extracellular matrix

heart regeneration

Angel Raya
contact affiliation	Center of Regenerative Medicine in Barcelona (CMRB)
contact email	araya@cmrb.eu
lab head
Francesc Canals
contact affiliation	Vall Hebron Institute of Oncology
contact email	fcanals@vhio.net
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/08/PXD010092

PRIDE project URI

• PRIDE
  1. PXD010092
     1. Label: PRIDE project
     2. Name: Proteomic analysis of extracellular matrix remodeling during zebrafish heart regeneration