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PXD010088

PXD010088 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleTransformation-induced stress at telomeres is counteracted through changes in the telomeric proteome including SAMHD1
DescriptionTelomeres play crucial roles during tumorigenesis inducing cellular senescence upon telomere shortening and extensive chromosome instability during telomere crisis. However, it has not been investigated if and how cellular transformation and oncogenic stress alters telomeric chromatin composition and function. Here we transform human fibroblasts by consecutive transduction with vectors expressing hTERT, the SV40 early region and activated H-RasV12. Pairwise comparisons of the telomeric proteome during different stages of transformation reveals upregulation of proteins involved in chromatin remodeling, DNA repair and replication at chromosome ends. Depletion of several of these proteins induces telomere fragility indicating their roles in replication of telomeric DNA. Depletion of SAMHD1, which has reported roles in DNA resection and homology directed repair, leads to telomere breakage events in cells deprived of the shelterin component TRF1. Thus our analysis identifies factors, which accumulate at telomeres during cellular transformation to promote telomere replication and repair, resisting oncogene-borne telomere replication stress.
HostingRepositoryPRIDE
AnnounceDate2018-07-13
AnnouncementXMLSubmission_2018-11-22_01:11:35.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterRomain Hamelin
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListphosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-06-11 04:44:24ID requested
12018-07-13 08:14:14announced
22018-11-22 01:11:36announcedUpdated publication reference for PubMed record(s): 30456372.
Publication List
Majerska J, Feretzaki M, Glousker G, Lingner J, Transformation-induced stress at telomeres is counteracted through changes in the telomeric proteome including SAMHD1. Life Sci Alliance, 1(4):e201800121(2018) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: Quantitative proteomics, replication stress, SAMHD1, telomeres, transformation
Contact List
Joachim Lingner
contact affiliationEcole Polytechnique Fédérale de Lausanne (EPFL), School of Life Sciences, 1015 Lausanne, Switzerland. Swiss Institute for Experimental Cancer Research (ISREC) at EPFL, Station 19, CH-1015 Lausanne, Switzerland
contact emailjoachim.lingner@epfl.ch
lab head
Romain Hamelin
contact affiliationPCF
contact emailromain.hamelin@epfl.ch
dataset submitter
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PRIDE project URI
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