PXD010085

PXD010085 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of genes regulated by miR-4510 in hepatocellular carcinoma
Description	Patients with advanced hepatocellular carcinoma (HCC) are currently treated by Sorafenib and Regorafenib but efficacy of these multikinase inhibitors is disappointing. Thus, new drugs and therapies are needed to improve HCC management in clinic. We recently reported the remarkable capacity of miR-4510 to impede the growth of HCC and hepatoblastoma cells in vitro and in vivo through GPC3 targeting and Wnt pathway inactivation (Cartier F et al, Oncotarget 2017). Here, we used a label-free proteomic approach to identify new targets of miR-4510 in HCC-deriving Huh7 cells. We transfected HCC-derived Huh7 cells with either a synthetic miR-4510 mimic or a small RNA control, extracted total proteins 24hr later and comparatively analyzed proteomes of control and miR-4510-transfected cells using a quantitative label-free approach.
HostingRepository	PRIDE
AnnounceDate	2019-10-23
AnnouncementXML	Submission_2019-10-25_03:57:47.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dupuy Jean-William
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-06-11 02:34:20	ID requested
1	2019-10-23 07:02:12	announced
⏵ 2	2019-10-25 03:57:49	announced	2019-10-25: Updated publication reference for PubMed record(s): 31612616.

Ghousein A, Mosca N, Cartier F, Charpentier J, Dupuy JW, Raymond AA, Bioulac-Sage P, Grosset CF, miR-4510 blocks hepatocellular carcinoma development through RAF1 targeting and RAS/RAF/MEK/ERK signalling inactivation. Liver Int, 40(1):240-251(2020) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: Liver, cancer, Hepatocellular carcinoma, label-free quantitative proteome analysis, miR-4510

Grosset Christophe
contact affiliation	Univ. Bordeaux, Inserm, BMGIC, U1035, 33076 Bordeaux, France.
contact email	christophe.grosset@inserm.fr
lab head
Dupuy Jean-William
contact affiliation	Plateforme Proteome Bordeaux
contact email	jean-william.dupuy@u-bordeaux.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/10/PXD010085

PRIDE project URI

• PRIDE
  1. PXD010085
     1. Label: PRIDE project
     2. Name: Proteomic analysis of genes regulated by miR-4510 in hepatocellular carcinoma