PXD010081

PXD010081 is an original dataset announced via ProteomeXchange.

Title	FA-SAT ncRNA interactome .
Description	FA-SAT is a satellite DNA sequence present and transcribed in many Bilateria species, what may anticipate a conserved and significant function in these genomes. Here we prove that in cat and human cells, FA-SAT satellite transcripts play a nuclear function at the G1 phase of the cell cycle. We identified and demonstrated that the main FA-SAT non-coding RNA interactor is the PKM2 protein. Our work shows that the disruption of the FA-SAT ncRNA/PKM2 protein complex, by the depletion of either FA-SAT or PKM2, results in the same phenotype—apoptosis. Moreover, the ectopic overexpression of FA-SAT in tumour human cells did not affect the cell cycle progression. In sum, our data reveal a new player, FA-SAT RNA, a non-coding satellite RNA, which interacts with the PKM2 nuclear protein. This ribonucleoprotein is involved in apoptosis and cell cycle progression, what foresees a promising new target for studies in cancer processes that rely on these pathways.
HostingRepository	PRIDE
AnnounceDate	2024-10-07
AnnouncementXML	Submission_2024-10-07_10:15:09.440.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sandra Anjo
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913; scientific name: Felis silvestris; NCBI TaxID: 9683; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidoethyl-L-cysteine
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2018-06-11 01:34:47	ID requested
⏵ 1	2024-10-07 10:15:15	announced

10.1007/s00018-019-03234-x;

Ferreira D, Escudeiro A, Adega F, Anjo SI, Manadas B, Chaves R, a switch from cell proliferation to apoptosis. Cell Mol Life Sci, 77(7):1371-1386(2020) [pubmed]

curator keyword: Biological

submitter keyword: FA-SAT

satellite DNA

non-coding RNA

Apoptosis

Interactome

SWATH-MS

Bruno Manadas
contact affiliation	Center for Neuroscience and Cell Biology – University of Coimbra
contact email	bmanadas@gmail.com
lab head
Sandra Anjo
contact affiliation	​Center for Neuroscience and Cell Biology – University of Coimbra
contact email	sandra.isabel.anjo@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/03/PXD010081

PRIDE project URI

• PRIDE
  1. PXD010081
     1. Label: PRIDE project
     2. Name: FA-SAT ncRNA interactome