PXD010073

PXD010073 is an original dataset announced via ProteomeXchange.

Title	Affinify Purification of EZH2 and phosphodeficient EZH2 identifies phosphorylation dependent interactions of EZH2
Description	Overexpression of EZH2 in estrogen receptor negative (ER-) breast cancer promotes metastasis. EZH2 has been mainly studied as the catalytic component of the Polycomb Repressive Complex 2 (PRC2) that mediates gene repression by trimethylating histone H3 at lysine 27 (H3K27me3). However, how EZH2 drives metastasis despite the low H3K27me3 levels observed in ER- breast cancer is unknown. We have shown that in human invasive carcinomas and distant metastases, cytoplasmic EZH2 phosphorylated at T367 is significantly associated with ER- disease and low H3K27me3 levels. Here, we explore the interactome of EZH2 and of a phosphodeficient mutant EZH2_T367A. We identified novel interactors of EZH2, and identified interactions that are dependent on the phosphorylation and cellular localization of EZH2 that may play a role in EZH2 dependent metastatic progression.
HostingRepository	PRIDE
AnnounceDate	2018-07-03
AnnouncementXML	Submission_2018-07-03_07:54:14.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	James Ropa
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-06-08 05:52:40	ID requested
⏵ 1	2018-07-03 07:54:15	announced

Dataset with its publication pending

curator keyword: Biological

submitter keyword: EZH2, phosphorylation, affinity purification, interactome, cellular localization

Celina Kleer
contact affiliation	Department of Pathology, University of Michigan
contact email	kleer@med.umich.edu
lab head
James Ropa
contact affiliation	University of Michigan
contact email	jropa@umich.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD010073
     1. Label: PRIDE project
     2. Name: Affinify Purification of EZH2 and phosphodeficient EZH2 identifies phosphorylation dependent interactions of EZH2