PXD010062

PXD010062 is an original dataset announced via ProteomeXchange.

Title	The proteome of pig spermatozoa is remodelled during ejaculation
Description	Proteins are essential for sperm function, including fertilizing capacity. Pig spermatozoa, emitted in well-defined ejaculate fractions, clearly vary in their functionality, which would be related to differences in protein composition. This study aimed firstly to update the porcine sperm proteome and secondly to identify proteins differentially expressed among mature spermatozoa from cauda epididymis and those fortuitously delivered in different ejaculate portions. Nine ejaculates from nine mature and fertile boars were manually collected in three separate ejaculate portions: the first 10 mL of sperm-rich ejaculate fraction (SRF), the rest of SRF and the post-SRF. The contents of cauda epididymides of the same boars was collected post-mortem by perfusion. All samples were centrifuged, pooling the resulting sperm pellets within the respective source-sample, which were later split to generate two technical replicates per source. The final eight sperm samples were subjected to iTRAQ-based 2D-LC-MS/MS for protein identification and quantification. A total of 1,723 proteins were identified (974 of Sus Scrofa taxonomy) and 1,602 of them were also quantified (960 of Sus Scrofa taxonomy). After an ANOVA test, 32 Sus Scrofa proteins showed quantitative differences (P < 0.01) among the sperm samples, which were particularly relevant for the functionality of spermatozoa fortuitously ejaculated in the post-SRF. The present study is the first showing quantitative differences in the protein profile of mature spermatozoa, involving proteins clearly implicated in sperm function, that prove the protein profile of boar spermatozoa is remodelled during ejaculation . These findings provide a valuable groundwork for further studies focused on identifying protein biomarkers of sperm fertility.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:45:51.443.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Luz Valero
SpeciesList	scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825;
ModificationList	biotinylated residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2018-06-07 07:47:29	ID requested
1	2018-10-02 06:02:09	announced
2	2018-10-04 00:30:27	announced	Updated project metadata.
⏵ 3	2024-10-22 04:45:55	announced	2024-10-22: Updated project metadata.

P, é, rez-Pati, ñ, o C, Parrilla I, Li J, Barranco I, Mart, í, nez EA, Rodriguez-Mart, í, nez H, Roca J, The Proteome of Pig Spermatozoa Is Remodeled During Ejaculation. Mol Cell Proteomics, 18(1):41-50(2019) [pubmed]

10.1074/mcp.ra118.000840;

curator keyword: Biological

submitter keyword: iTRAQ

artificial insemination

seminal plasma

sperm-rich ejaculate fraction;

Jordi Roca
contact affiliation	Department of Medicine and Animal Surgery, Faculty of Veterinary Science, University of Murcia, Spain
contact email	roca@um.es
lab head
Luz Valero
contact affiliation	Universitat de Valencia
contact email	mluz.valero@uv.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD010062

PRIDE project URI

• PRIDE
  1. PXD010062
     1. Label: PRIDE project
     2. Name: The proteome of pig spermatozoa is remodelled during ejaculation