PXD010045 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Quantitative proteomics of potato leaves infected withPhytophthora infestans provides insights into coordinated and altered protein expression during early and late disease stages in Solanum tuberosum (cv. Sarpo Mira) |
Description | Potato (Solanum tuberosum) late blight disease caused by Phytophthora. infestans is one of the most critical crop diseases in the world. We investigated the proteome of Solanum tubersoum (cv. Sarpo Mira) by quantitative proteomics after foliar application of encysted zoospore suspension from P. infestans isolate. Also, we examined changes in protein abundance of cv. Sarpo Mira in response to P. infestans infection at three key timepoints 0 hpi (L), 48 hpi (M) and 120 hpi (H), divided into early and late disease stages and analyzed the protein-protein interaction during the disease stages. In response to P. infestans oomycete infection, the iTRAQ comparative proteome survey revealed a total of 1229 differentially expressed proteins (DEPs) in the pairwise comparison of disease stages, which included DEPs specific to early disease stage, DEPs specific to late disease stage and commonly shared DEPs. Over 80% of the change in protein abundance were up-regulated in the early stages of infection while about 61% of the DEPs in the later disease stage were down-regulated. The analysis of expression patterns, functional category, and enrichment tests highlighted significant coordination and enrichment of cell wall-associated defense response proteins in the early stages and cellular protein modification process as well as membrane protein complex formation in the late stages. Furthermore, strong protein interactions were observed in the late disease stage that might relate to disease pathogenesis. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:47:37.010.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | qian zhou |
SpeciesList | scientific name: Solanum tuberosum (Potato); NCBI TaxID: 4113; |
ModificationList | TMT6plex-126 reporter+balance reagent acylated residue |
Instrument | LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-06-06 07:15:08 | ID requested | |
1 | 2019-01-09 02:58:44 | announced | |
⏵ 2 | 2024-10-22 04:47:37 | announced | 2024-10-22: Updated project metadata. |
Publication List
Xiao C, Gao J, Zhang Y, Wang Z, Zhang D, Chen Q, Ye X, Xu Y, Yang G, Yan L, Cheng Q, Chen J, Shen Y, Provides Insights into Coordinated and Altered Protein Expression during Early and Late Disease Stages. Int J Mol Sci, 20(1):(2019) [pubmed] |
10.3390/ijms20010136; |
Keyword List
curator keyword: Biological |
submitter keyword: Late blight, Phytophthora infestans,Proteome, Potato, Early and Late disease stages., Sarpo Mira |
Contact List
Chunfang Xiao |
contact affiliation | Hu Bei province,enshi Acandemy of Agricutural sciences |
contact email | xiaochunfang22@163.com |
lab head | |
qian zhou |
contact affiliation | China |
contact email | zhouqian@omicsspace.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD010045
- Label: PRIDE project
- Name: Quantitative proteomics of potato leaves infected withPhytophthora infestans provides insights into coordinated and altered protein expression during early and late disease stages in Solanum tuberosum (cv. Sarpo Mira)