PXD010045

PXD010045 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteomics of potato leaves infected withPhytophthora infestans provides insights into coordinated and altered protein expression during early and late disease stages in Solanum tuberosum (cv. Sarpo Mira)
Description	Potato (Solanum tuberosum) late blight disease caused by Phytophthora. infestans is one of the most critical crop diseases in the world. We investigated the proteome of Solanum tubersoum (cv. Sarpo Mira) by quantitative proteomics after foliar application of encysted zoospore suspension from P. infestans isolate. Also, we examined changes in protein abundance of cv. Sarpo Mira in response to P. infestans infection at three key timepoints 0 hpi (L), 48 hpi (M) and 120 hpi (H), divided into early and late disease stages and analyzed the protein-protein interaction during the disease stages. In response to P. infestans oomycete infection, the iTRAQ comparative proteome survey revealed a total of 1229 differentially expressed proteins (DEPs) in the pairwise comparison of disease stages, which included DEPs specific to early disease stage, DEPs specific to late disease stage and commonly shared DEPs. Over 80% of the change in protein abundance were up-regulated in the early stages of infection while about 61% of the DEPs in the later disease stage were down-regulated. The analysis of expression patterns, functional category, and enrichment tests highlighted significant coordination and enrichment of cell wall-associated defense response proteins in the early stages and cellular protein modification process as well as membrane protein complex formation in the late stages. Furthermore, strong protein interactions were observed in the late disease stage that might relate to disease pathogenesis.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:47:37.010.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	qian zhou
SpeciesList	scientific name: Solanum tuberosum (Potato); NCBI TaxID: 4113;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2018-06-06 07:15:08	ID requested
1	2019-01-09 02:58:44	announced
⏵ 2	2024-10-22 04:47:37	announced	2024-10-22: Updated project metadata.

Xiao C, Gao J, Zhang Y, Wang Z, Zhang D, Chen Q, Ye X, Xu Y, Yang G, Yan L, Cheng Q, Chen J, Shen Y, Provides Insights into Coordinated and Altered Protein Expression during Early and Late Disease Stages. Int J Mol Sci, 20(1):(2019) [pubmed]

10.3390/ijms20010136;

curator keyword: Biological

submitter keyword: Late blight, Phytophthora infestans,Proteome, Potato, Early and Late disease stages., Sarpo Mira

Chunfang Xiao
contact affiliation	Hu Bei province,enshi Acandemy of Agricutural sciences
contact email	xiaochunfang22@163.com
lab head
qian zhou
contact affiliation	China
contact email	zhouqian@omicsspace.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/01/PXD010045

PRIDE project URI

• PRIDE
  1. PXD010045
     1. Label: PRIDE project
     2. Name: Quantitative proteomics of potato leaves infected withPhytophthora infestans provides insights into coordinated and altered protein expression during early and late disease stages in Solanum tuberosum (cv. Sarpo Mira)