PXD010042

PXD010042 is an original dataset announced via ProteomeXchange.

Title	In search of Varroa chemosensory related proteins using transcriptomic and proteomic tools
Description	The tight synchronization between the life cycle of the obligatory parasitic mite Varroa destructor (Varroa) and its host the honeybee, is mediated by honeybee chemical stimuli. These stimuli are mainly perceived by a pit organ located at the distal part of the mite’s foreleg. In the present study, we searched for Varroa chemosensory molecular components by comparing transcriptomic and proteomic profiles between forelegs from different physiological stages, and rear legs. In general, a comparative transcriptomic analysis showed a clear separation of the expression profiles between the rear legs and the three groups of forelegs (phoretic, reproductive and tray-collected mites). Most of the differentially expressed transcripts and proteins in the mite’s foreleg were previously uncharacterized. Using a conserved domain approach, we identified 54 transcripts with known chemosensory-related domains, of which 17 were significantly up regulated in the mite’s forelegs when compared to rear legs. We characterized the chemosensory transcripts using phylogenetic analysis.
HostingRepository	PRIDE
AnnounceDate	2018-11-21
AnnouncementXML	Submission_2018-11-21_05:17:57.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD010042
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Meital Kupervaser
SpeciesList	scientific name: Varroa destructor; NCBI TaxID: 109461;
ModificationList	Deamidated; Oxidation; Carbamidomethyl
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-06-05 08:50:48	ID requested
⏵ 1	2018-11-21 05:17:58	announced

Eliash N, Thangarajan S, Goldenberg I, Sela N, Kupervaser M, Barlev J, Altman Y, Knyazer A, Kamer Y, Zaidman I, Rafaeli A, Soroker V, Varroa chemosensory proteins: some are conserved across Arthropoda but others are arachnid specific. Insect Mol Biol, 28(3):321-341(2019) [pubmed]

curator keyword: Biological

submitter keyword: Varroa mites, honeybee, forelegs, odorant binding proteins

Victoria Soroker
contact affiliation	1Institute of Plant Protection, Agricultural Research Organization, The Volcani Center, Rishon LeZion, Israel
contact email	sorokerv@volcani.agri.gov.il
lab head
Meital Kupervaser
contact affiliation	Weizmann Institute of Science
contact email	meital.kupervaser@weizmann.ac.il
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/11/PXD010042

PRIDE project URI

• PRIDE
  1. PXD010042
     1. Label: PRIDE project
     2. Name: In search of Varroa chemosensory related proteins using transcriptomic and proteomic tools