PXD010040

PXD010040 is an original dataset announced via ProteomeXchange.

Title	Comparative interactome analyses of wild-type and P301L MAPT in two human neuronal cell lines
Description	Protein interactions of the tau protein are of interest in efforts to decipher the mechanisms of cell death in Alzheimer Disease (AD), a subset of frontotemporal dementias (FTD) and other tauopathies. We recently reported on extensive interactions of tau with the ribonucleoproteome and chaperones. A confounder of this prior work was that it probed steady-state interactions of plasmid-encoded four-repeat (4R) tau in dividing cells. Since then, we genome-edited a genomic safe harbor locus in two human neuronal cell lines, namely IMR-32 and ReNcell VM, creating inducible EGFP tagged wild-type and P301L tau models. We expressed balanced levels of 3R- and 4R-tau and interrogated tau protein interactions at specific times following its induction. In addition to its association with the ribonucleoproteome, tau was observed to interact in these models with proteins that escaped prior investigations.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:45:39.209.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Declan Williams
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-pyridylethyl-L-cysteine; phosphorylated residue; iTRAQ8plex-116 reporter+balance reagent acylated residue; monohydroxylated residue; deamidated residue
Instrument	Orbitrap Fusion ETD

Revision	Datetime	Status	ChangeLog Entry
0	2018-06-05 08:18:58	ID requested
1	2019-11-12 23:36:26	announced
⏵ 2	2024-10-22 04:45:46	announced	2024-10-22: Updated project metadata.

10.1038/s41598-019-52543-5;

Wang X, Williams D, M, ü, ller I, Lemieux M, Dukart R, Maia IBL, Wang H, Woerman AL, Schmitt-Ulms G, Tau interactome analyses in CRISPR-Cas9 engineered neuronal cells reveal ATPase-dependent binding of wild-type but not P301L Tau to non-muscle myosins. Sci Rep, 9(1):16238(2019) [pubmed]

curator keyword: Biological

submitter keyword: tauopathy, neuron,Alzheimer disease, human, frontotemporal dementia, inducible expression, orbitrap, tau, interactome, MAPT

Gerold Schmitt-Ulms
contact affiliation	Tanz Centre for Research in Neurodegenerative Diseases, Faculty of Medicine, University of Toronto
contact email	g.schmittulms@utoronto.ca
lab head
Declan Williams
contact affiliation	University of Toronto
contact email	gwillym.williams@utoronto.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/11/PXD010040

PRIDE project URI

• PRIDE
  1. PXD010040
     1. Label: PRIDE project
     2. Name: Comparative interactome analyses of wild-type and P301L MAPT in two human neuronal cell lines