PXD010036

PXD010036 is an original dataset announced via ProteomeXchange.

Title	Analysis of proteins in supernatant and precipitate fractions of human bronchial smooth muscle cells by 1D SDS-PAGE and quantitative LC-MS/MS
Description	Supernatant and precipitate protein fractions of human bronchial smooth muscle cells (HBSMC) after sonication and centrifugation were analyzed using 1D SDS-PAGE. The gel lanes were cube-cut into thirty-three 1.1 mm x 1.1 mm x 1 mm (gel thickness) squares and each gel piece was subjected to in-gel digestion and quantitative LC-MS/MS. Totally 2552 proteins were assigned from the lane of supernatant and 2614 protein species from the lane of precipitate. The results are being further analyzed, e.g. 1) for the comparison between the proteins species residing in the two fractions, and 2) for correlation with the nondenaturing 2DE-grid cut-quantitative LC-MS/MS results (Jin, et al. Electrophoresis 2015, 36, 1711-1723, Jin, et al. Electrophoresis 2015, 1991-2001, PRIDE project PXD001471).
HostingRepository	PRIDE
AnnounceDate	2019-01-03
AnnouncementXML	Submission_2019-01-03_02:12:42.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ya Jin
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2018-06-05 07:22:05	ID requested
⏵ 1	2019-01-03 02:12:43	announced

Jin Y, Zhang J, Manabe T, Tan W, Comparison of the performance of 1D SDS-PAGE with nondenaturing 2DE on the analysis of proteins from human bronchial smooth muscle cells using quantitative LC-MS/MS. J Chromatogr B Analyt Technol Biomed Life Sci, 1105():193-202(2019) [pubmed]

curator keyword: Biological

submitter keyword: bronchial smooth muscle cells, supernatant fraction, precipitation fraction, 1D SDS-PAGE, quantitative LC-MS/MS

Ya Jin
contact affiliation	Institute of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou, 511400, P. R. China
contact email	yajin@gdut.edu.cn
lab head
Ya Jin
contact affiliation	Institute of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology
contact email	ya.jin@ymail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/01/PXD010036

PRIDE project URI

• PRIDE
  1. PXD010036
     1. Label: PRIDE project
     2. Name: Analysis of proteins in supernatant and precipitate fractions of human bronchial smooth muscle cells by 1D SDS-PAGE and quantitative LC-MS/MS