PXD010034

PXD010034 is an original dataset announced via ProteomeXchange.

Title	Origin and evolution of neural microexons
Description	The mechanisms by which entire programs of gene regulation emerged during evolution are poorly understood. Neuronal microexons represent the most conserved class of alternative splicing in vertebrates and are critical for proper brain development and function. Here, we discover neural microexon programs in non-vertebrate species and trace their origin to bilaterian ancestors through the emergence of a previously uncharacterized ‘enhancer of microexons' (eMIC) protein domain. The eMIC domain originated as an alternative, neural-enriched splice isoform of the pan-eukaryotic Srrm2/SRm300 splicing factor gene, and subsequently became fixed in the vertebrate and neuronal-specific splicing regulator Srrm4/nSR100 and its paralog Srrm3. Remarkably, the eMIC domain is necessary and sufficient for microexon splicing, and functions by interacting with the earliest components required for exon recognition. The emergence of a novel domain with restricted expression in the nervous system thus resulted in the evolution of splicing programs that contributed to qualitatively expand neuronal molecular complexity in bilaterians.
HostingRepository	PRIDE
AnnounceDate	2019-03-12
AnnouncementXML	Submission_2019-03-11_18:24:33.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Antonio Torres Mendez
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2018-06-05 02:57:30	ID requested
⏵ 1	2019-03-11 18:24:34	announced

Torres-M, é, ndez A, Bonnal S, Marquez Y, Roth J, Iglesias M, Permanyer J, Almud, í I, O'Hanlon D, Guitart T, Soller M, Gingras AC, Gebauer F, Rentzsch F, Blencowe BJ, Valc, á, rcel J, Irimia M, A novel protein domain in an ancestral splicing factor drove the evolution of neural microexons. Nat Ecol Evol, 3(4):691-701(2019) [pubmed]

curator keyword: Biological

submitter keyword: human, nuclear extract, ribonucleoprotein complex, microexon

Manuel Irimia Martinez
contact affiliation	Centre for Genomic Regulation, Barcelona Institute of Science and Technology (BIST), Barcelona 08003, Spain
contact email	mirimia@gmail.com
lab head
Antonio Torres Mendez
contact affiliation	Centre for Genomic Regulation
contact email	antonio.torres@crg.eu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/03/PXD010034

PRIDE project URI

• PRIDE
  1. PXD010034
     1. Label: PRIDE project
     2. Name: Origin and evolution of neural microexons