PXD010024

PXD010024 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	An unprecedented binding mode of vitamin B12 in the predicted transporter BtuM
Description	BtuM binds vitamin B12 (cyano-cobalamin) in an unprecedented way. Structural and spectroscopic data indicate that the unusual thiolate coordination allows for chemical modification of the substrate, which is decyanation of the compund. We use mass-spectrometry to show the loss of the 26 Da moiety with various BtuM versions and cyano-cobalamin and dicyano-cobinamide.
HostingRepository	PRIDE
AnnounceDate	2018-10-18
AnnouncementXML	Submission_2018-10-18_03:01:13.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Stephan Rempel
SpeciesList	scientific name: Thiobacillus denitrificans ATCC 25259; NCBI TaxID: 292415;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2018-06-04 05:47:35	ID requested
⏵ 1	2018-10-18 03:01:15	announced

Publication List

Rempel S, Colucci E, de Gier JW, Guskov A, Slotboom DJ, Cysteine-mediated decyanation of vitamin B12 by the predicted membrane transporter BtuM. Nat Commun, 9(1):3038(2018) [pubmed]

Rempel S, Colucci E, de Gier JW, Guskov A, Slotboom DJ, Cysteine-mediated decyanation of vitamin B12 by the predicted membrane transporter BtuM. Nat Commun, 9(1):3038(2018) [pubmed]

Keyword List

curator keyword: Biological
submitter keyword: BtuM, decyanation

curator keyword: Biological

submitter keyword: BtuM, decyanation

Contact List

Dirk Jan Slotboom
contact affiliation	1Groningen Biomolecular and Biotechnology Institute (GBB), University of Groningen, 9474AG Groningen, The Netherlands 2Zernike Institute for Advanced Materials, University of Groningen, 9747AG Groningen, The Netherlands
contact email	d.j.slotboom@rug.nl
lab head
Stephan Rempel
contact affiliation	University of Groningen
contact email	s.r.rempel@rug.nl
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD010024
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD010024

PRIDE project URI

Repository Record List

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