PXD010020

PXD010020 is an original dataset announced via ProteomeXchange.

Title	Associating H2O2 and NO related changes in the proteome of Mycobacterium smegmatis with enhanced survival in macrophages
Description	In this study, we used mass spectrometry-based discovery proteomic analysis to examine the cell-wide changes of Mycobacterium smegmatis, in response to sub-lethal concentrations of H2O2 and nitric oxide (NO) over time. Preliminary results have shown that sub-lethal concentrations of reactive oxygen and nitrogen species may act as signalling molecules by playing a role in the overall protein abundance that may be conducive to survival in vivo. The presented study showed that both treatments induced: the DosR regulon, alterations in protein expression and lipid metabolism. In addition to the proteomic changes observed, we have also observed increased survival after macrophage infection of Mycobacterium smegmatis pre-exposed to sub-lethal doses of H2O2 or DETA-NO. ¬ In total, 27 samples yielded approximately 3300 M. smegmatis protein group identifications. More in-depth analysis of this new dataset will provide a better understanding as to the inner workings of mycobacteria under oxidative and nitrosative stress and potential mechanisms by which exposure to sub-lethal concentrations of stressors results in an increased resistance to stress.
HostingRepository	PRIDE
AnnounceDate	2019-11-12
AnnouncementXML	Submission_2019-11-12_11:23:55.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Bridget Calder
SpeciesList	scientific name: Mycobacterium smegmatis; NCBI TaxID: 1772;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-06-04 03:44:00	ID requested
⏵ 1	2019-11-12 11:23:57	announced

Ganief N, Sjouerman J, Albeldas C, Nakedi KC, Hermann C, Calder B, Blackburn JM, Soares NC, and NO-related changes in the proteome of Mycobacterium smegmatis with enhanced survival in macrophage. Emerg Microbes Infect, 7(1):212(2018) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: ROS, H2O2, NO, Signalling, Proteomics, sub-lethal concentrations, Mycobacterium

Jonathan Blackburn
contact affiliation	Integrative biomedical sciences, Health Sciences Campus, University of Cape Town, South Africa
contact email	jonathan.blackburn@uct.ac.za
lab head
Bridget Calder
contact affiliation	University of Cape Town
contact email	bridget.calder@uct.ac.za
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD010020
     1. Label: PRIDE project
     2. Name: Associating H2O2 and NO related changes in the proteome of Mycobacterium smegmatis with enhanced survival in macrophages