PXD010018

PXD010018 is an original dataset announced via ProteomeXchange.

Title	Cherry Adapted Plum Pox Virus (PPV) CR Isolate Alters the Abundance of Proteins Involved in Photosynthesis and Stress-response of the Experimental Herbaceous Host Nicotiana benthamiana More Aggressively than the Mild PPV-C Isolate
Description	Plum pox virus (PPV, family Potyviridae) is one of the most important viral pathogens of Prunus spp. causing considerable damage to stone-fruit industry worldwide each year. Among the PPV strains identified so far, only PPV-C and PPV-CR are able to infect cherries under natural conditions. Herein, we evaluated the impact of strains differing by the pathogenic potential on herbaceous host Nicotiana benthamiana. At first glance, a faster accumulation of PPV capsid protein was noticed by semi-quantitative DAS-ELISA on tobacco leaves infected by PPV-CR (the RU-30sc isolate) in contrast to PPV-C (BY-101 isolate). This result was correlated perfectly with observed phenotypic symptoms. To assess the host response to infection more deeply, a comprehensive proteomic profiling was performed using reverse phase UHPLC followed by label-free mass spectrometry quantification. Thirty-one unique plant proteins were identified as significantly altered due to the infection. Precise evaluation of particular amount shifts of identified proteins in relation to infection has revealed that the aggressive PPV-CR isolate has a stronger influence on the abundance of photosynthesis-related proteins, mainly from Calvin cycle, as the mild PPV-C. This observation was accompanied by a significant reduction of photosynthetic pigments extracted from the leaves of PPV-CR infected plants. Shifts in the abundance of remaining identified proteins, indicates activation of repair mechanism, stimulation of photosynthetic capacity, and modifications in amino acid and carbohydrate metabolism to affect plant growth and initiate energy formation via gluconeogenesis. Furthermore, we speculate that accumulation of H2O2, in PPV-CR infected leaves may activate glutathione synthesis, which plays a crucial role in further plant defense and development.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:45:51.470.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD010018
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Maksym Danchenko
SpeciesList	scientific name: Nicotiana benthamiana; NCBI TaxID: 4100; scientific name: Plum pox virus; NCBI TaxID: 12211;
ModificationList	Deamidated; Oxidation; Carbamidomethyl
Instrument	Q-Tof Premier

Revision	Datetime	Status	ChangeLog Entry
0	2018-06-04 02:26:20	ID requested
1	2018-08-13 11:10:58	announced
⏵ 2	2024-10-22 04:45:55	announced	2024-10-22: Updated project metadata.

10.6019/PXD010018;

Nov, á, kov, á S, Danchenko M, Skultety L, Fialov, á I, Le, š, kov, á A, Beke G, Flores-Ram, í, rez G, Glasa M, Photosynthetic and Stress Responsive Proteins Are Altered More Effectively in Nicotiana benthamiana Infected with Plum pox virus Aggressive PPV-CR versus Mild PPV-C Cherry-Adapted Isolates. J Proteome Res, 17(9):3114-3127(2018) [pubmed]

10.1021/acs.jproteome.8b00230;

curator keyword: Biological

submitter keyword: tobacco, LC-MS, compatible interaction, photosynthesis,sharka, PPV, label-free quantification, antioxidants

Slavomira Novakova
contact affiliation	Biomedical Research Center, Slovak Academy of Sciences
contact email	viruslno@savba.sk
lab head
Maksym Danchenko
contact affiliation	Plant Science and Biodiversity Center SAS
contact email	maxymdan@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD010018

PRIDE project URI

• PRIDE
  1. PXD010018
     1. Label: PRIDE project
     2. Name: Cherry Adapted Plum Pox Virus (PPV) CR Isolate Alters the Abundance of Proteins Involved in Photosynthesis and Stress-response of the Experimental Herbaceous Host Nicotiana benthamiana More Aggressively than the Mild PPV-C Isolate