PXD010009

PXD010009 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	SamavarchiTehrani_P112_VS4_Viral_BioID_Benchmark_human
Description	This submission contains the mass spectrometry files for the manuscript by Samavarchi-Tehrani et al. that describes the use of lentiviral delivery of BirA*-tagged transgenes into HeLa, BJ and MEF cells or in comparison to stably integrated transgene in Flp-In T-REx HeLa cell lines. This submission contains the human files from a set of 56 raw MS files and associated peak lists and result files, acquired on a TripleTOF 6600 spectrometer (AB SCIEX, Concord, Ontario, Canada) operated in Data Dependent Acquisition mode. Samples were generated by Payman Samavarchi-Tehrani. This massIVE submission contains the files for the mouse samples. Please see accompanying submission for the human samples. Raw files are deposited here in MassIVE. For questions, please contact Anne-Claude Gingras (gingras@lunenfeld.ca) or Payman Samavarchi-Tehrani (payman@lunenfeld.ca). Table 1 describes the composition of this dataset Table 2 lists all the peptide identification evidence (as per iProphet) Table 3 lists the SAINTexpress interactions Internal reference from the Gingras lab ProHits implementation: Project 112, Export versions VS4 (Viral BioID Benchmark) SAINT Task ID: Flp-HeLa:3596; HeLa:3139; BJ:3140; MEF:3141
HostingRepository	MassIVE
AnnounceDate	2018-08-03
AnnouncementXML	Submission_2018-08-03_07:28:16.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Gingras Lab
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	deamidated L-asparagine; deamidated L-glutamine; L-methionine sulfoxide
Instrument	TripleTOF 6600

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2018-06-01 14:32:27	ID requested
⏵ 1	2018-08-03 07:28:16	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: Affinity purification, BioID, Proximity-dependent biotinylation, Protein-protein interaction, AP-MS, Lentivirus

submitter keyword: Affinity purification, BioID, Proximity-dependent biotinylation, Protein-protein interaction, AP-MS, Lentivirus

Contact List

Anne-Claude Gingras
contact affiliation	LTRI
contact email	gingras@lunenfeld.ca
lab head
Gingras Lab
contact affiliation	Samuel Lunenfeld Research Institute
contact email	gingras@mshri.on.ca
dataset submitter

Full Dataset Link List

MassIVE dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000082430

MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000082430