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PXD010008

PXD010008 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSamavarchiTehrani_P112_VS4_Viral_BioID_Benchmark_mouse
DescriptionThis submission contains the mass spectrometry files for the manuscript by Samavarchi-Tehrani et al. that describes the use of lentiviral delivery of BirA*-tagged transgenes into HeLa, BJ and MEF cells or in comparison to stably integrated transgene in Flp-In T-REx HeLa cell lines. This submission contains the mouse files from a set of 56 raw MS files and associated peak lists and result files, acquired on a TripleTOF 6600 spectrometer (AB SCIEX, Concord, Ontario, Canada) operated in Data Dependent Acquisition mode. Samples were generated by Payman Samavarchi-Tehrani. This massive submission contains the files for the mouse samples. Please see accompanying submission for the human samples. Raw files are deposited here in MassIVE. For questions, please contact Anne-Claude Gingras (gingras@lunenfeld.ca) or Payman Samavarchi-Tehrani (payman@lunenfeld.ca). Table 1 describes the composition of this dataset Table 2 lists all the peptide identification evidence (as per iProphet) Table 3 lists the SAINTexpress interactions Internal reference from the Gingras lab ProHits implementation: Project 112, Export versions VS4 (Viral BioID Benchmark) SAINT Task ID: Flp-HeLa:3596; HeLa:3139; BJ:3140; MEF:3141
HostingRepositoryMassIVE
AnnounceDate2018-08-03
AnnouncementXMLSubmission_2018-08-03_07:31:00.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterGingras Lab
SpeciesList scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090;
ModificationListdeamidated L-asparagine; deamidated L-glutamine; L-methionine sulfoxide
InstrumentTripleTOF 6600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-06-01 14:19:47ID requested
12018-08-03 07:31:01announced
Publication List
Samavarchi-Tehrani P, Abdouni H, Samson R, Gingras AC, A Versatile Lentiviral Delivery Toolkit for Proximity-dependent Biotinylation in Diverse Cell Types. Mol Cell Proteomics, 17(11):2256-2269(2018) [pubmed]
Keyword List
submitter keyword: Affinity purification, BioID, Proximity-dependent biotinylation, Protein-protein interaction, AP-MS, Lentivirus
Contact List
Anne-Claude Gingras
contact affiliationLTRI
contact emailgingras@lunenfeld.ca
lab head
Gingras Lab
contact affiliationSamuel Lunenfeld Research Institute
contact emailgingras@mshri.on.ca
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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