PXD010006 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Characterization of the phospho-signalling mediated by the sodium-potassium ATPase in response to ouabain |
Description | In this project we applied a workflow for phosphoproteomics analysis to characterize the phosphorylation signalling that is triggered by the cardiotonic steroid ouabain. Ouabain is a ligand for the sodium-potassium ATPase (NKA). Low concentration of ouabain trigger oscillation of intracellular calcium concentration and modulate the phosphorylation of several signaling molecules, impacting cellular proliferation, apoptosis and cell-to-cell contacts. Monkey kidney cells (COS-7) were either treated for 10 or 20 minutes with 100 nM ouabain, or left untreated. We identify 15,348 phospho-sites, of which 1,941 are significantly regulated at either one of the time points employed. Analysis of the significantly regulated phospho-sites shed light on the molecular mechanisms by which ouabain affects cell junctions, proliferation and apoptosis. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:45:52.533.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Elena Panizza |
SpeciesList | scientific name: Chlorocebus sabaeus; NCBI TaxID: 60711; |
ModificationList | phosphorylated residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-06-01 08:45:03 | ID requested | |
1 | 2019-09-13 00:19:46 | announced | |
⏵ 2 | 2024-10-22 04:45:57 | announced | 2024-10-22: Updated project metadata. |
Publication List
Panizza E, Zhang L, Fontana JM, Hamada K, Svensson D, Akkuratov EE, Scott L, Mikoshiba K, Brismar H, Lehti, ö J, Aperia A, -ATPase control of cell adhesion, proliferation, and survival. FASEB J, 33(9):10193-10206(2019) [pubmed] |
10.1096/fj.201900445R; |
Keyword List
curator keyword: Biological |
submitter keyword: phosphoproteomics, IP3R, Akt, MAPK, calcium-calmodulin-dependent protein kinase, nka, InsP3 receptor, calcium, ouabain, na-k-atpase, sodium-potassium ATPase, apoptosis, calcium oscillations, CAMK |
Contact List
Anita Aperia |
contact affiliation | Department of Women and Children’s Health, Science for Life Laboratory, Karolinska Institutet, Stockholm, Sweden |
contact email | Anita.Aperia@ki.se |
lab head | |
Elena Panizza |
contact affiliation | Cornell University |
contact email | elena.panizza84@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD010006
- Label: PRIDE project
- Name: Characterization of the phospho-signalling mediated by the sodium-potassium ATPase in response to ouabain