PXD010006

PXD010006 is an original dataset announced via ProteomeXchange.

Title	Characterization of the phospho-signalling mediated by the sodium-potassium ATPase in response to ouabain
Description	In this project we applied a workflow for phosphoproteomics analysis to characterize the phosphorylation signalling that is triggered by the cardiotonic steroid ouabain. Ouabain is a ligand for the sodium-potassium ATPase (NKA). Low concentration of ouabain trigger oscillation of intracellular calcium concentration and modulate the phosphorylation of several signaling molecules, impacting cellular proliferation, apoptosis and cell-to-cell contacts. Monkey kidney cells (COS-7) were either treated for 10 or 20 minutes with 100 nM ouabain, or left untreated. We identify 15,348 phospho-sites, of which 1,941 are significantly regulated at either one of the time points employed. Analysis of the significantly regulated phospho-sites shed light on the molecular mechanisms by which ouabain affects cell junctions, proliferation and apoptosis.
HostingRepository	PRIDE
AnnounceDate	2019-09-13
AnnouncementXML	Submission_2019-09-13_00:19:44.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Elena Panizza
SpeciesList	scientific name: Chlorocebus sabaeus; NCBI TaxID: 60711;
ModificationList	phosphorylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-06-01 08:45:03	ID requested
⏵ 1	2019-09-13 00:19:46	announced

Panizza E, Zhang L, Fontana JM, Hamada K, Svensson D, Akkuratov EE, Scott L, Mikoshiba K, Brismar H, Lehti, ö J, Aperia A, -ATPase control of cell adhesion, proliferation, and survival. FASEB J, 33(9):10193-10206(2019) [pubmed]

curator keyword: Biological

submitter keyword: phosphoproteomics, ouabain, calcium oscillations, apoptosis, na-k-atpase, nka, sodium-potassium ATPase, calcium, Akt, MAPK, IP3R, InsP3 receptor, CAMK, calcium-calmodulin-dependent protein kinase

Anita Aperia
contact affiliation	Department of Women and Children’s Health, Science for Life Laboratory, Karolinska Institutet, Stockholm, Sweden
contact email	Anita.Aperia@ki.se
lab head
Elena Panizza
contact affiliation	Cornell University
contact email	elena.panizza84@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/09/PXD010006

PRIDE project URI

• PRIDE
  1. PXD010006
     1. Label: PRIDE project
     2. Name: Characterization of the phospho-signalling mediated by the sodium-potassium ATPase in response to ouabain