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PXD009987

PXD009987 is an original dataset announced via ProteomeXchange.

Dataset Summary
Titlequantitative proteomic analysis of phagocytes in oyster Crassostrea gigas
DescriptionInvertebrates lack the adaptive immune system homologous to that of vertebrates, thus depend exceptionally on the innate immune system for the pathogen recognition and clearance. Phagocytes play a pivotal role in the innate immunity of invertebrates, but the molecular basis underlying their phagocytic killing is still far from well understood. In the present study, phagocytes from the Pacific oyster Crassostrea gigas were efficiently sorted based on the phagocytosis of FITC-labeled latex beads by fluorescence-activated cell sorting. The differentially expressed proteins were revealed by quantitative proteome and validated by qRT-PCR and flow cytometry.
HostingRepositoryiProX
AnnounceDate2018-05-29
AnnouncementXMLSubmission_2019-06-17_20:57:51.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterShuai Jiang
SpeciesList scientific name: Crassostrea gigas; NCBI TaxID: 29159;
ModificationListiTRAQ8plex reporter+balance reagent acylated residue; iodoacetamide derivatized residue; monohydroxylated residue
InstrumentTripleTOF 5600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-05-31 20:10:10ID requested
12018-05-31 20:10:23announced
22019-06-17 20:57:53announcedUpdate publication information.
Publication List
Jiang S, Qiu L, Wang L, Jia Z, Lv Z, Wang M, Liu C, Xu J, Song L, . Front Immunol, 9():1280(2018) [pubmed]
Keyword List
submitter keyword: quantitative proteomics, phagocytes, Crassostrea gigas
Contact List
Linsheng Song
contact affiliationLiaoning Key Laboratory of Marine Animal Immunology & Disease Control, Dalian Ocean University
contact emaillshsong@dlou.edu.cn
lab head
Shuai Jiang
contact affiliationInstitute of Oceanology Chinese Academy of Sciences
contact emailsjiang@qdio.ac.cn
dataset submitter
Full Dataset Link List
iProX dataset URI