PXD009979 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | SWATH quantitative proteomics and cellular assays show in vitro anticancer activity of ARA- and DHA-based monoacylglycerols in colorectal cancer cells |
Description | Colorectal cancer is one of the most common and widespread disease in the world and the third type of cancer causing a high mortality rate. There is increasing evidence that some polyunsaturated fatty acids (PUFAs) are involved in the reduction of cancer risk and progression. Recent studies showed that sn-2 monoacylglycerols (MAG) exercise specific inhibitory actions on cancer cells through different mechanisms. However, the anticancer effect of PUFA-based MAGs on colorectal cancer has yet to be assessed. In this work we investigated the actions of two PUFAs, docosahexaenoic (DHA, 22: 6n3) and arachidonic acids (ARA, 20:4n6), both as MAG, on colon cancer human (HT-29) cell line. We performed the MTT test, LDH, and caspase-3 assays, while global proteome changes were assessed by SWATH-MS quantitative proteomics followed by pathway analysis in order to find out which molecular mechanisms were being affected. It has been proven that ARA- and DHA-MAG exercises dose- and time-dependent antiproliferative actions. In all cases, DHA-MAG acts on cancer cells more efficiently than ARA-MAG. Results clearly demonstrate the ability of MAGs to induce cell death in colon cancer cells and suggest a direct relationship between their chemical structure and their potency. Therefore, sn-2 MAG are suitable candidate for the production of new functional ingredients. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:49:53.650.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Ignacio Ortea |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | TripleTOF 5600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-05-31 03:12:31 | ID requested | |
1 | 2022-01-28 05:15:52 | announced | |
⏵ 2 | 2023-11-14 08:49:54 | announced | 2023-11-14: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
curator keyword: Biomedical |
submitter keyword: monoacylglycerol,colorectal cancer, SWATH |
Contact List
Ignacio Ortea |
contact affiliation | Proteomics Unit, IMIBIC, Reina Sofía University Hospital, University of Córdoba, Córdoba, Spain |
contact email | ignacio.ortea@imibic.org |
lab head | |
Ignacio Ortea |
contact affiliation | IMIBIC |
contact email | iortea@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD009979
- Label: PRIDE project
- Name: SWATH quantitative proteomics and cellular assays show in vitro anticancer activity of ARA- and DHA-based monoacylglycerols in colorectal cancer cells