PXD009979

PXD009979 is an original dataset announced via ProteomeXchange.

Title	SWATH quantitative proteomics and cellular assays show in vitro anticancer activity of ARA- and DHA-based monoacylglycerols in colorectal cancer cells
Description	Colorectal cancer is one of the most common and widespread disease in the world and the third type of cancer causing a high mortality rate. There is increasing evidence that some polyunsaturated fatty acids (PUFAs) are involved in the reduction of cancer risk and progression. Recent studies showed that sn-2 monoacylglycerols (MAG) exercise specific inhibitory actions on cancer cells through different mechanisms. However, the anticancer effect of PUFA-based MAGs on colorectal cancer has yet to be assessed. In this work we investigated the actions of two PUFAs, docosahexaenoic (DHA, 22: 6n3) and arachidonic acids (ARA, 20:4n6), both as MAG, on colon cancer human (HT-29) cell line. We performed the MTT test, LDH, and caspase-3 assays, while global proteome changes were assessed by SWATH-MS quantitative proteomics followed by pathway analysis in order to find out which molecular mechanisms were being affected. It has been proven that ARA- and DHA-MAG exercises dose- and time-dependent antiproliferative actions. In all cases, DHA-MAG acts on cancer cells more efficiently than ARA-MAG. Results clearly demonstrate the ability of MAGs to induce cell death in colon cancer cells and suggest a direct relationship between their chemical structure and their potency. Therefore, sn-2 MAG are suitable candidate for the production of new functional ingredients.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:49:53.650.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ignacio Ortea
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2018-05-31 03:12:31	ID requested
1	2022-01-28 05:15:52	announced
⏵ 2	2023-11-14 08:49:54	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

curator keyword: Biomedical

submitter keyword: monoacylglycerol,colorectal cancer, SWATH

Ignacio Ortea
contact affiliation	Proteomics Unit, IMIBIC, Reina Sofía University Hospital, University of Córdoba, Córdoba, Spain
contact email	ignacio.ortea@imibic.org
lab head
Ignacio Ortea
contact affiliation	IMIBIC
contact email	iortea@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD009979
     1. Label: PRIDE project
     2. Name: SWATH quantitative proteomics and cellular assays show in vitro anticancer activity of ARA- and DHA-based monoacylglycerols in colorectal cancer cells