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PXD009978

PXD009978 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomic analysis of separated Golgi cisternae gives insights into secretory pathway organization Proteomic analysis of separated Golgi cisternae gives insights into secretory pathway organization
DescriptionThe Golgi apparatus is the hub of the secretory pathway, trafficking proteins and lipids and synthesizing complex glycans. The spatial distribution of resident proteins across individual Golgi cisternae is functionally essential for complex molecule biosynthesis. However, the inability to-date to separate Golgi cisternae means both the distribution of most residents, and the mechanisms determining their distribution, are unknown. Here, we exploit differences in surface charge between membranes to perform the first separation of Golgi cisternae. We localize over 400 proteins to the Golgi and describe the cisternal distribution of over 250 Golgi residents, as well as complex glycans. Both protein and glycan distributions are validated in-vivo using super-resolution microscopy. Results reveal distinct functional compartmentalization amongst resident Golgi proteins, which relates to the distribution of carbohydrate reaction products. Analysis of cisternal proteomes shows that exoplasmic protein pI, exoplasmic hydrophobicity, Ser content and asymmetric Phe distribution increase across the Golgi.
HostingRepositoryPRIDE
AnnounceDate2024-10-07
AnnouncementXMLSubmission_2024-10-07_10:15:02.567.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD009978
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterHarriet Parsons
SpeciesList scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationListOxidation; Carbamidomethyl
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-05-31 03:04:53ID requested
12024-10-07 10:15:03announced
Publication List
10.6019/PXD009978;
Parsons HT, Stevens TJ, McFarlane HE, Vidal-Melgosa S, Griss J, Lawrence N, Butler R, Sousa MML, Salemi M, Willats WGT, Petzold CJ, Heazlewood JL, Lilley KS, Reveals Underlying Properties of Cisternal Localization. Plant Cell, 31(9):2010-2034(2019) [pubmed]
10.1105/tpc.19.00081;
Keyword List
curator keyword: Biological
submitter keyword: Proteomic analysis of separated Golgi cisternae gives insights into secretory pathway organization
Contact List
Harriet T Parsons
contact affiliationCambridge University
contact emailtempeparsons@gmail.com
lab head
Harriet Parsons
contact affiliationCambridge University
contact emailtempeparsons@gmail.com
dataset submitter
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Dataset FTP location
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