PXD009976

PXD009976 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Nucleotide pyrophosphatase/phosphodiesterase from Euphorbia characias latex
Description	The low-resolution structure of a nucleotide pyrophosphatase/phosphodiesterase from Euphorbia characias latex (ELNPP) has been determined in solution by means of Small Angle X-ray Scattering (SAXS). To improve the structural resolution of ELNPP a partial sequencing after proteolytic cleavage of the protein was performed. Protein digestion followed by high-resolution HPLC-ESI-MS/MS analysis allowed us to identify two different peptides of 82 and 41 amino acids, respectively. These sequences exhibit a high degree of identity with other NPPs predicted sequences from several higher plants including Malus domestica, Morus notabilis, Ricinus communis, and Triticum aestivum. In particular, Triticum aestivum NPP is the protein with the highest identity level (98 identical positions) towards the obtained fragments of ELNPP. From the alignment with the entire sequence of TaNPP, the two obtained fragments contain some known conserved residues belonging to the catalytic domain of the mammalian NPP family enzymes.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:34:51.531.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tiziana Cabras
SpeciesList	scientific name: Euphorbia characias; NCBI TaxID: 3991;
ModificationList	phosphorylated residue
Instrument	LTQ Orbitrap Elite

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2018-05-31 01:58:00	ID requested
1	2022-03-02 01:48:03	announced
⏵ 2	2024-10-22 05:34:52	announced	2024-10-22: Updated project metadata.

Publication List

10.1107/s2059798320010207;
Sabatucci A, Pintus F, Cabras T, Vincenzoni F, Maccarrone M, Medda R, Dainese E, Structure of a nucleotide pyrophosphatase/phosphodiesterase (NPP) from Euphorbia characias latex characterized by small-angle X-ray scattering: clues for the general organization of plant NPPs. Acta Crystallogr D Struct Biol, 76(Pt 9):857-867(2020) [pubmed]

10.1107/s2059798320010207;

Sabatucci A, Pintus F, Cabras T, Vincenzoni F, Maccarrone M, Medda R, Dainese E, Structure of a nucleotide pyrophosphatase/phosphodiesterase (NPP) from Euphorbia characias latex characterized by small-angle X-ray scattering: clues for the general organization of plant NPPs. Acta Crystallogr D Struct Biol, 76(Pt 9):857-867(2020) [pubmed]

Keyword List

curator keyword: Biological
submitter keyword: Nucleotide pyrophosphatase/phosphodiesterase,Euphorbia characias, MSMS sequencing

curator keyword: Biological

submitter keyword: Nucleotide pyrophosphatase/phosphodiesterase,Euphorbia characias, MSMS sequencing

Contact List

Tiziana Cabras
contact affiliation	University of Cagliari Dep. Life and Environmental Sciences
contact email	tcabras@unica.it
lab head
Tiziana Cabras
contact affiliation	University of Cagliari, DISVA
contact email	tcabras@unica.it
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/03/PXD009976
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/03/PXD009976

PRIDE project URI

Repository Record List

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