PXD009975

PXD009975 is an original dataset announced via ProteomeXchange.

Title	Proximity biotinylation and LC-MSMS identification of the molecular microenvironment of coronavirus replication complexes
Description	To investigate virus-host interactions at the site of coronavirus replication, we developed a biotin-based proximity labelling approach by engineering a promiscuous biotin ligase, BirA-R118G, within the coronavirus replication and transcription complex (RTC). BirA-R118G was fused to non-structural protein 2 to generate MHV- BirA-R118G-nsp2. During the course of infection, viral and host factors in proximity of the viral RTC become covalently biotinylated, allowing affinity purification of biotin-tagged factors and mass spectrometry identification of RTC-proximal viral and host factors. Results provide a comprehensive library of RTC-associated factors which likely include crucial factors that promote, orchestrate and assist viral replication within the viral RTC microenvironment.
HostingRepository	PRIDE
AnnounceDate	2019-01-16
AnnouncementXML	Submission_2019-01-16_00:08:56.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Manfred Heller
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Murine hepatitis virus strain A59; NCBI TaxID: 11142;
ModificationList	biotinylated residue; monohydroxylated residue; acetylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2018-05-31 01:32:19	ID requested
⏵ 1	2019-01-16 00:08:58	announced

V'kovski P, Gerber M, Kelly J, Pfaender S, Ebert N, Braga Lagache S, Simillion C, Portmann J, Stalder H, Gaschen V, Bruggmann R, Stoffel MH, Heller M, Dijkman R, Thiel V, Determination of host proteins composing the microenvironment of coronavirus replicase complexes by proximity-labeling. Elife, 8():(2019) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: L929 mouse fibroblast, mouse hepatitis virus, viral replication complex, BioID, BirA-R118G , LC-MSMS

Volker Thiel
contact affiliation	Institute of Virology and Immunology IVI, Bern, Switzerland; Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Bern, Switzerland
contact email	volker.thiel@vetsuisse.unibe.ch
lab head
Manfred Heller
contact affiliation	University of Bern
contact email	manfred.heller@dbmr.unibe.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/01/PXD009975

PRIDE project URI

• PRIDE
  1. PXD009975
     1. Label: PRIDE project
     2. Name: Proximity biotinylation and LC-MSMS identification of the molecular microenvironment of coronavirus replication complexes