PXD009971

PXD009971 is an original dataset announced via ProteomeXchange.

Title	Identification of hypoxia-induced proteins using SILAC-based proteomics
Description	A hallmark of solid tumours is the development of hypoxic regions where rapidly growing transformed cells outstrip their blood supply. Tumour cells in these starved regions sense reduced levels of oxygen and switch on genes that help them to adapt, survive and continue growing. Since hypoxia is a key physiological difference between normal and cancer tissue, an opportunity exists to selectively kill tumour cells by exploiting the differences in protein expression between normal and hypoxic tumour tissue. However, a major challenge is to identify druggable hypoxia-induced proteins critical for tumour cell survival. This is especially important in cancers of unmet need where hypoxia gene signatures correlate with poor prognosis (for example, colorectal cancers). To identify new hypoxia-induced proteins, we performed SILAC-based proteomics on colorectal cancer cells exposed to normoxia and hypoxia. In this study, we identify a new hypoxia-activated GPCR signalling axis that enables colorectal tumour cells to survive the microenvironmental stress of hypoxia. Our findings uncover a previously unappreciated role for this GPCR-axis as a key regulator of the adaptive response to hypoxia and highlght an opportunity to exploit tumour-associated hypoxia for therapy.
HostingRepository	PRIDE
AnnounceDate	2018-09-18
AnnouncementXML	Submission_2018-09-18_06:46:23.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alexander Greenhough
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2018-05-30 16:27:56	ID requested
⏵ 1	2018-09-18 06:46:23	announced

Dataset with its publication pending

curator keyword: Biological, Biomedical

submitter keyword: Colorectal cancer, hypoxia, SILAC

Ann C. Williams
contact affiliation	Biomedical Sciences, University of Bristol
contact email	ann.c.williams@bristol.ac.uk
lab head
Alexander Greenhough
contact affiliation	University of Bristol
contact email	a.greenhough@bristol.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/09/PXD009971

PRIDE project URI

• PRIDE
  1. PXD009971
     1. Label: PRIDE project
     2. Name: Identification of hypoxia-induced proteins using SILAC-based proteomics