PXD009967

PXD009967 is an original dataset announced via ProteomeXchange.

Title	Identification of novel ejaculated seminal fluid proteins in a seed beetle and division of labour across male accessory reproductive glands
Description	The male ejaculate contains a multitude of seminal fluid proteins (SFPs), many of which are key reproductive molecules. However, the identification of SFPs is notoriously difficult and a detailed understanding of this complex phenotype has only been achieved in a few model species. We employed a recently developed proteomic method involving whole-organism stable isotopeic labelling coupled with proteomic and transcriptomic analyses which allowed us to successfully characterize male SFPs in the seed beetle Callosobruchus maculatus. We identified 317 proteins that were transferred to females at mating, and a great majority of these showed signals of secretion and were significantly male-biased in expression in the abdomen. Male SFPs were enriched with proteins involved in general metabolic and catabolic processes but also with proteolytic enzymes and proteins involved in protection against oxidative stress. Thirty-seven proteins showed significant homology with SFPs previously identified in other insects. However, no less than 92 C. maculatus SFPs were entirely novel, receiving no significant blast hits and lacking homologs in extant data bases, consistent with a rapid and divergent evolution of SFPs. In a second effort, we used 3D micro-tomography in conjunction with proteomic methods to identify 5 distinct pairs of male accessory reproductive glands and to show that certain male SFPs are only produced in certain male glands. We suggest that this division of labour may allow males to tailor ejaculate composition. Finally, we provide a tentative list of 231 candidate female-derived reproductive proteins, some of which are likely important in ejaculate processing and/or sperm storage.
HostingRepository	PRIDE
AnnounceDate	2025-06-28
AnnouncementXML	Submission_2025-06-28_06:50:00.788.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Helen Bayram
SpeciesList	scientific name: Callosobruchus maculatus; NCBI TaxID: 64391;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-05-30 15:34:33	ID requested
⏵ 1	2025-06-28 06:50:01	announced

10.1016/j.ibmb.2018.12.002;

Bayram H, Sayadi A, Immonen E, Arnqvist G, Identification of novel ejaculate proteins in a seed beetle and division of labour across male accessory reproductive glands. Insect Biochem Mol Biol, 104():50-57(2019) [pubmed]

curator keyword: Biological

submitter keyword: novel proteins, accessory sex glands,Ejaculate, seed beetle

Goran Arnqvist
contact affiliation	Department of Ecology and Genetics, Uppsala University
contact email	Goran.Arnqvist@ebc.uu.se
lab head
Helen Bayram
contact affiliation	University of Manchester
contact email	helen.bayram@manchester.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD009967
     1. Label: PRIDE project
     2. Name: Identification of novel ejaculated seminal fluid proteins in a seed beetle and division of labour across male accessory reproductive glands