PXD009960

PXD009960 is an original dataset announced via ProteomeXchange.

Title	(TMT)-quantitative proteomic study of Bacillus cereus tsu1
Description	In this project, we report the bacterial proteome expression profile change for different time points when culturing in rapeseed cake substrate (RCS). Bacillus cereus tsu1 was cultured in RCS (25g/L) for 12 hours, 24 hours and 48 hours. At each time point, cells were stained with Sudan Black to monitor PHB accumulation. Bacterial cells were loaded with PHB after 12-hour culture and significant degradation of PHB was observed after 48-hour culture. Bacterial proteome profile changes were identified using tandem mass tags mass spectrometry (TMT-MS)-based quantitative proteomics analysis. From TMT proteomics analysis, 3,237 proteins were detected and quantified from the bacterial pellet protein extraction, from which proteins related with PHB biosynthesis and degradation were identified. Quantitative analysis revealed that 146 (between 12h and 24h samples), and 158 (between 12h and 48h) proteins showed significant differences. Several STRING protein interaction networks were developed for significantly changed protein related with sporulation and anaerobic respiration.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:25:17.953.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ted Thannhauser
SpeciesList	scientific name: Bacillus cereus; NCBI TaxID: 1396;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2018-05-30 03:55:45	ID requested
1	2021-09-08 06:59:00	announced
⏵ 2	2024-10-22 05:25:20	announced	2024-10-22: Updated project metadata.

10.1186/s12866-020-01815-6;

Li H, O'Hair J, Thapa S, Bhatti S, Zhou S, Yang Y, Fish T, Thannhauser TW, Proteome profile changes during poly-hydroxybutyrate intracellular mobilization in gram positive Bacillus cereus tsu1. BMC Microbiol, 20(1):122(2020) [pubmed]

curator keyword: Biological

submitter keyword: PHB pathway proteins,TMT-quantitative proteomics

protein profile of Bacillus cereus tsu1

Theodore Thannhauser
contact affiliation	USDA-ARS
contact email	tt34@cornell.edu
lab head
Ted Thannhauser
contact affiliation	USDA-ARS
contact email	tt34@cornell.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD009960

PRIDE project URI

• PRIDE
  1. PXD009960
     1. Label: PRIDE project
     2. Name: (TMT)-quantitative proteomic study of Bacillus cereus tsu1