PXD009952

PXD009952 is an original dataset announced via ProteomeXchange.

Title	Maximally-multiplexed proteome quantification platform for isotopic metabolic and chemical labeling
Description	Quantitative proteomic platforms based on precursor intensity in mass spectrometry (MS1-level) uniquely support in vivo metabolic labeling with superior quantification accuracy but suffers from limited multiplexity (≤3-plex) and frequent missing quantities. Herein, we present a maximally-multiplexed MS1-level quantification platform that comprises new six-plex in vivo SILAC or in vitro di-ethylation with a dedicated algorithm. We demonstrate accurate and missing quantity-free quantification of highly complex samples with broad dynamic ranges. With our platform, we globally profile the proteome dynamics under the heat shock response of HeLa cells.
HostingRepository	PRIDE
AnnounceDate	2020-03-19
AnnouncementXML	Submission_2020-03-19_07:52:23.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yeon Choi
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-05-29 02:34:22	ID requested
⏵ 1	2020-03-19 07:52:25	announced

Choi Y, Jeong K, Shin S, Lee JW, Lee YS, Kim S, Kim SA, Jung J, Kim KP, Kim VN, Kim JS, Chemical Labeling. Anal Chem, 92(7):4980-4989(2020) [pubmed]

submitter keyword: Protein Quantification, Quantification, SILAC, Diethylation, SILAC-6plex, DE-6plex, Heat shock response, Heat shock, HeLa cell

Jong-Seo Kim
contact affiliation	Center for RNA Research, Institute for Basic Science, Seoul 08826, Korea School of Biological Sciences, Seoul National University, Seoul 08826, Korea
contact email	jongseokim@snu.ac.kr
lab head
Yeon Choi
contact affiliation	Seoul National University
contact email	kitechoi9599@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/03/PXD009952

PRIDE project URI

• PRIDE
  1. PXD009952
     1. Label: PRIDE project
     2. Name: Maximally-multiplexed proteome quantification platform for isotopic metabolic and chemical labeling