PXD009948

PXD009948 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Comprehensive ADP-ribosylome analysis identifies tyrosine as an ADP-ribose acceptor site
Description	Despite recent mass spectrometry (MS)-based breakthroughs, comprehensive ADP-ribose (ADPr)-site identification and localization remain challenging. Here, we report the establishment of an unbiased, multistep ADP-ribosylome data analysis workflow that led to the identification of tyrosine as a novel ARTD1/PARP1 dependent in vivo ADPr-acceptor. MS analyses of in vitro ADP-ribosylated proteins confirmed tyrosine as an ADPr-acceptor amino acid in RPS3A (Y155) and HPF1 (Y238) and demonstrated that trans modification of RPS3A is dependent on HPF1. We provide an ADPr-site Localization Spectra Database (ADPr-LSD), which contains 288 high-quality ADPr-modified peptide spectra, to serve as ADPr spectral references for correct ADPr-site localizations.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:06:37.434.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD009948
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Michael Hottiger
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Oxidation; Carbamidomethyl
Instrument	Q Exactive HF; Orbitrap Fusion

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2018-05-29 02:27:08	ID requested
1	2018-06-28 08:28:58	announced
2	2018-07-30 01:26:31	announced	Updated publication reference for PubMed record(s): 29954836.
⏵ 3	2024-10-22 04:06:38	announced	2024-10-22: Updated project metadata.

Publication List

10.6019/PXD009948;
10.15252/embr.201745310;
Leslie Pedrioli DM, Leutert M, Bilan V, Nowak K, Gunasekera K, Ferrari E, Imhof R, Malmstr, ö, m L, Hottiger MO, Comprehensive ADP-ribosylome analysis identifies tyrosine as an ADP-ribose acceptor site. EMBO Rep, 19(8):(2018) [pubmed]

10.6019/PXD009948;

10.15252/embr.201745310;

Leslie Pedrioli DM, Leutert M, Bilan V, Nowak K, Gunasekera K, Ferrari E, Imhof R, Malmstr, ö, m L, Hottiger MO, Comprehensive ADP-ribosylome analysis identifies tyrosine as an ADP-ribose acceptor site. EMBO Rep, 19(8):(2018) [pubmed]

Keyword List

curator keyword: Biological
submitter keyword: Tyrosine ADP-ribosylation, PARP1, ARTD1, HPF1, RSP3A, Genotoxic stress,ADP-ribosylation

curator keyword: Biological

submitter keyword: Tyrosine ADP-ribosylation, PARP1, ARTD1, HPF1, RSP3A, Genotoxic stress,ADP-ribosylation

Contact List

Michael O. Hottiger
contact affiliation	Department of Molecular Mechanisms of Disease, University of Zurich, CH-8057 Zurich, Switzerland
contact email	michael.hottiger@dmmd.uzh.ch
lab head
Michael Hottiger
contact affiliation	UZH
contact email	dmmd.admin@dmmd.uzh.ch
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD009948
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD009948

PRIDE project URI

Repository Record List

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