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PXD009947

PXD009947 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSerum Small Extracellular Vesicles Proteome of Tuberculosis Patients Demonstrated Deregulated Immune Response.
DescriptionDetailed understanding of host pathogen interaction in tuberculosis is an important avenue for identifying novel therapeutic targets. Small extracellular vesicles (EVs) like exosomes are rich in proteins, nucleic acids and lipids, act as messenger and may show altered composition in disease condition. In this case control study, we isolated small EVs from serum of 58 subjects (33 (15-70) in years) including drug naïve active tuberculosis (ATB: n=22), non-tuberculosis (NTB: n=18) and healthy subjects (n=18). Serum small EVs proteome analysis was carried out using isobaric tag for relative and absolute quantification (iTRAQ) experiments and an independent sample set (n=42) was used for validation. A set of 132 and 68 proteins were identified in iTRAQ-I (ATB/Healthy) and iTRAQ-II (ATB/NTB) experiments respectively. Four proteins (KYAT3, SERPINA1, HP and APOC3) showed deregulation (log2 fold change >±0.48 at p<0.05) in ATB with respect to healthy controls and Western blot data corroborated mass spectrometry findings. These important proteins involve in kynurenine metabolic pathway, neutrophil degranulation, scavenging of heme from plasma and lipid metabolism showed deregulation in ATB patients. Identification of such a protein panel in circulating small EVs besides providing novel insights into their role in tuberculosis may prove to be useful targets to develop host-directed therapeutic intervention.
HostingRepositoryMassIVE
AnnounceDate2020-01-06
AnnouncementXMLSubmission_2020-01-06_07:49:59.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterRakesh Arya
SpeciesList scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationListunknown modification
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-05-29 02:15:20ID requested
12018-05-29 02:18:43announced
22020-01-06 07:50:01announced2019-11-08: Adding publication [PMID31532894] to dataset [MSV000082412]
Publication List
Arya R, Dabral D, Faruquee HM, Mazumdar H, Patgiri SJ, Deka T, Basumatary R, Kupa RU, Semy C, Kapfo W, Liegise K, Kaur I, Choedon T, Kumar P, Behera RK, Deori P, Nath R, Khalo K, Saikia L, Khamo V, Nanda RK, Serum Small Extracellular Vesicles Proteome of Tuberculosis Patients Demonstrated Deregulated Immune Response. Proteomics Clin Appl, 14(1):e1900062(2020) [pubmed]
Keyword List
submitter keyword: tuberculosis, exosomes, proteomics, iTRAQ, LC-MS/MS
Contact List
Dr. Ranjan Kumar Nanda
contact affiliationInternational Center for Genetic Engineering and Biotechnology, New Delhi, INDIA
contact emailranjan@icgeb.res.in
lab head
Rakesh Arya
contact affiliationICGEB
contact emailrakesharya@icgeb.res.in
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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