PXD009947
PXD009947 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Serum Small Extracellular Vesicles Proteome of Tuberculosis Patients Demonstrated Deregulated Immune Response. |
Description | Detailed understanding of host pathogen interaction in tuberculosis is an important avenue for identifying novel therapeutic targets. Small extracellular vesicles (EVs) like exosomes are rich in proteins, nucleic acids and lipids, act as messenger and may show altered composition in disease condition. In this case control study, we isolated small EVs from serum of 58 subjects (33 (15-70) in years) including drug naïve active tuberculosis (ATB: n=22), non-tuberculosis (NTB: n=18) and healthy subjects (n=18). Serum small EVs proteome analysis was carried out using isobaric tag for relative and absolute quantification (iTRAQ) experiments and an independent sample set (n=42) was used for validation. A set of 132 and 68 proteins were identified in iTRAQ-I (ATB/Healthy) and iTRAQ-II (ATB/NTB) experiments respectively. Four proteins (KYAT3, SERPINA1, HP and APOC3) showed deregulation (log2 fold change >±0.48 at p<0.05) in ATB with respect to healthy controls and Western blot data corroborated mass spectrometry findings. These important proteins involve in kynurenine metabolic pathway, neutrophil degranulation, scavenging of heme from plasma and lipid metabolism showed deregulation in ATB patients. Identification of such a protein panel in circulating small EVs besides providing novel insights into their role in tuberculosis may prove to be useful targets to develop host-directed therapeutic intervention. |
HostingRepository | MassIVE |
AnnounceDate | 2020-01-06 |
AnnouncementXML | Submission_2020-01-06_07:49:59.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Rakesh Arya |
SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
ModificationList | unknown modification |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2018-05-29 02:15:20 | ID requested | |
1 | 2018-05-29 02:18:43 | announced | |
⏵ 2 | 2020-01-06 07:50:01 | announced | 2019-11-08: Adding publication [PMID31532894] to dataset [MSV000082412] |
Publication List
Arya R, Dabral D, Faruquee HM, Mazumdar H, Patgiri SJ, Deka T, Basumatary R, Kupa RU, Semy C, Kapfo W, Liegise K, Kaur I, Choedon T, Kumar P, Behera RK, Deori P, Nath R, Khalo K, Saikia L, Khamo V, Nanda RK, Serum Small Extracellular Vesicles Proteome of Tuberculosis Patients Demonstrated Deregulated Immune Response. Proteomics Clin Appl, 14(1):e1900062(2020) [pubmed] |
Keyword List
submitter keyword: tuberculosis, exosomes, proteomics, iTRAQ, LC-MS/MS |
Contact List
Dr. Ranjan Kumar Nanda | |
---|---|
contact affiliation | International Center for Genetic Engineering and Biotechnology, New Delhi, INDIA |
contact email | ranjan@icgeb.res.in |
lab head | |
Rakesh Arya | |
contact affiliation | ICGEB |
contact email | rakesharya@icgeb.res.in |
dataset submitter |
Full Dataset Link List
MassIVE dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000082412/ |