PXD009947

PXD009947 is an original dataset announced via ProteomeXchange.

Title	Serum Small Extracellular Vesicles Proteome of Tuberculosis Patients Demonstrated Deregulated Immune Response.
Description	Detailed understanding of host pathogen interaction in tuberculosis is an important avenue for identifying novel therapeutic targets. Small extracellular vesicles (EVs) like exosomes are rich in proteins, nucleic acids and lipids, act as messenger and may show altered composition in disease condition. In this case control study, we isolated small EVs from serum of 58 subjects (33 (15-70) in years) including drug naïve active tuberculosis (ATB: n=22), non-tuberculosis (NTB: n=18) and healthy subjects (n=18). Serum small EVs proteome analysis was carried out using isobaric tag for relative and absolute quantification (iTRAQ) experiments and an independent sample set (n=42) was used for validation. A set of 132 and 68 proteins were identified in iTRAQ-I (ATB/Healthy) and iTRAQ-II (ATB/NTB) experiments respectively. Four proteins (KYAT3, SERPINA1, HP and APOC3) showed deregulation (log2 fold change >±0.48 at p<0.05) in ATB with respect to healthy controls and Western blot data corroborated mass spectrometry findings. These important proteins involve in kynurenine metabolic pathway, neutrophil degranulation, scavenging of heme from plasma and lipid metabolism showed deregulation in ATB patients. Identification of such a protein panel in circulating small EVs besides providing novel insights into their role in tuberculosis may prove to be useful targets to develop host-directed therapeutic intervention.
HostingRepository	MassIVE
AnnounceDate	2020-01-06
AnnouncementXML	Submission_2020-01-06_07:49:59.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rakesh Arya
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	unknown modification
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2018-05-29 02:15:20	ID requested
1	2018-05-29 02:18:43	announced
⏵ 2	2020-01-06 07:50:01	announced	2019-11-08: Adding publication [PMID31532894] to dataset [MSV000082412]

Arya R, Dabral D, Faruquee HM, Mazumdar H, Patgiri SJ, Deka T, Basumatary R, Kupa RU, Semy C, Kapfo W, Liegise K, Kaur I, Choedon T, Kumar P, Behera RK, Deori P, Nath R, Khalo K, Saikia L, Khamo V, Nanda RK, Serum Small Extracellular Vesicles Proteome of Tuberculosis Patients Demonstrated Deregulated Immune Response. Proteomics Clin Appl, 14(1):e1900062(2020) [pubmed]

submitter keyword: tuberculosis, exosomes, proteomics, iTRAQ, LC-MS/MS

Dr. Ranjan Kumar Nanda
contact affiliation	International Center for Genetic Engineering and Biotechnology, New Delhi, INDIA
contact email	ranjan@icgeb.res.in
lab head
Rakesh Arya
contact affiliation	ICGEB
contact email	rakesharya@icgeb.res.in
dataset submitter

MassIVE dataset URI

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