PXD009946

PXD009946 is an original dataset announced via ProteomeXchange.

Title	Alternative digestion approaches improve histone modification mapping by mass spectrometry in clinical samples
Description	Profiling histone post-translational modifications (PTMs) in clinical samples holds great potential for the identification of epigenetic biomarkers and the discovery of novel epigenetic targets. We have recently developed a battery of mass spectrometry (MS)-based approaches to analyze histone PTMs in different types of primary samples. However, most of these protocols rely on SDS-PAGE separation following histone enrichment in order to eliminate detergents and isolate histones from other proteins present in the sample. As a consequence, many proteolytic enzymes, whose performance is poor in gel, cannot be used, limiting the digestions options for clinical samples, and hence the modification coverage. In this study, we used a simple procedure involving acetone protein precipitation followed by histone enrichment through a C18 StageTip column to obtain histone preparations suitable for various in solution digestion protocols.
HostingRepository	PRIDE
AnnounceDate	2021-03-04
AnnouncementXML	Submission_2021-03-04_12:43:59.777.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Roberta Noberini
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	methylated residue; acetylated residue; N6; dimethylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2018-05-29 01:32:33	ID requested
⏵ 1	2021-03-04 12:44:00	announced

Restellini C, Cuomo A, Lupia M, Giordano M, Bonaldi T, Noberini R, Alternative digestion approaches improve histone modification mapping by mass spectrometry in clinical samples. Proteomics Clin Appl, 13(1):e1700166(2019) [pubmed]

curator keyword: Technical, Biological, Biomedical

submitter keyword: histone post-translational modifications, epigenetics, proteomics

Tiziana Bonaldi
contact affiliation	European Institute of Oncology, Milan
contact email	tiziana.bonaldi@ieo.it
lab head
Roberta Noberini
contact affiliation	Istituto Europeo di Oncologia
contact email	roberta.noberini@ieo.it
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2010/10/PXD009946

PRIDE project URI

• PRIDE
  1. PXD009946
     1. Label: PRIDE project
     2. Name: Alternative digestion approaches improve histone modification mapping by mass spectrometry in clinical samples