PXD009938

PXD009938 is an original dataset announced via ProteomeXchange.

Title	Human THP1 macrophages after interacting with C. albicans, using ATP-binding protein enrichment strategy
Description	Macrophages are involved in the primary human host response to pathogens such as Candida albicans. After pathogen recognition by specific receptors, a cascade of signaling pathways is activated, leading to the production of cytokines, chemokines and antimicrobial peptides. ATP binding proteins are crucial for the regulation of signaling pathways. Taking into account the fact that proteomics can provide important information regarding these molecular mechanisms, a quantitative proteomic approach was carried out for the study of human macrophage ATP-binding proteins after interaction with C. albicans. A total of 547 non-redundant proteins were quantified in at least two biological replicates and with a standard deviation lower than 0.3. Among them, 59 proteins were considered differentially abundant during macrophage interaction with C. albicans. Moreover, with the proteins enriched in ATP-binding proteins a phosphopeptide enrichment was performed, and a total of 70 phosphosites was quantified, using the same filtering criteria. We repeated the search of the .raw files in the new nexprot database (NextProt v2.15.0, 20230 protein entries) that we also uploaded in this project. In summary, in this project we uploaded: a. Quantitative proteomic results- .raw files; .msf files; mgf and excel table with quantitative results b. Quantitative phosphor results - .raw files; .msf files; mgf and excel table with quantitative results c. Identification results with recent version of Nexprot-mzIdentML.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:06:10.509.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Concha Gil
SpeciesList	scientific name: Candida albicans (Yeast); NCBI TaxID: 5476;
ModificationList	phosphorylated residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2018-05-28 06:26:07	ID requested
1	2019-04-18 07:30:34	announced
⏵ 2	2024-10-22 04:06:11	announced	2024-10-22: Updated project metadata.

Vaz C, Reales-Calderon JA, Pitarch A, Vellosillo P, Trevisan M, Hern, á, ez ML, Monteoliva L, Gil C, Enrichment of ATP Binding Proteins Unveils Proteomic Alterations in Human Macrophage Cell Death, Inflammatory Response, and Protein Synthesis after Interaction with Candida albicans. J Proteome Res, 18(5):2139-2159(2019) [pubmed]

10.1021/acs.jproteome.9b00032;

curator keyword: Biological, Biomedical

submitter keyword: phosphopeptides, SILAC, ATP-binding proteins,Macrophages, Candida albicans

Concha Gil Garcia
contact affiliation	Complutense University of Madrid
contact email	conchagil@ucm.es
lab head
Concha Gil
contact affiliation	UCM
contact email	cai.proteomica@pas.ucm.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/04/PXD009938

PRIDE project URI

• PRIDE
  1. PXD009938
     1. Label: PRIDE project
     2. Name: Human THP1 macrophages after interacting with C. albicans, using ATP-binding protein enrichment strategy