PXD009915

PXD009915 is an original dataset announced via ProteomeXchange.

Title	Evidence for the nuclear import of monomeric histones H3.1 & H4
Description	We present here evidence that histones H3.1 and H4 can be imported into the nucleus as monomers in human cells. Using a tether-and-release system to study the cytosolic phase and import dynamics of newly synthesised histones, we find that H3.1 and H4 can be maintained as stable monomers in the cytosol in a tethered state. Cytosolically tethered histones are bound tightly to Importin- proteins (predominantly IPO4), but not to the histone specific chaperones NASP, ASF1a, RbAp46 (RBBP7) or HAT1, which reside in the nucleus in interphase cells. Release of monomeric histones from their cytosolic tether results in rapid nuclear translocation, dissociation with IPO4 and incorporation into chromatin at sites of replication. Quantitative analysis of histones bound to individual chaperones under steady-state conditions reveals an excess of H3 specifically associated with sNASP, suggesting that NASP can maintain a soluble, monomeric pool of H3 within the nucleus and may act as a nuclear receptor for newly imported histone. In summary, we propose that histones H3 and H4 are rapidly imported as monomeric units, forming heterodimers in the nucleus rather than the cytosol, with sNASP acting as a potential nuclear receptor for monomeric histone H3.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:06:15.973.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Juan Ramon Hernandez-Fernaud
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2018-05-25 07:39:28	ID requested
1	2018-09-10 03:29:42	announced
⏵ 2	2024-10-22 04:06:17	announced	2024-10-22: Updated project metadata.

Apta-Smith MJ, Hernandez-Fernaud JR, Bowman AJ, Evidence for the nuclear import of histones H3.1 and H4 as monomers. EMBO J, 37(19):(2018) [pubmed]

10.15252/embj.201798714;

curator keyword: Biological

submitter keyword: Human, histones

Andrew Bowman
contact affiliation	Warwick Medical School University of Warwick
contact email	A.Bowman.1@warwick.ac.uk
lab head
Juan Ramon Hernandez-Fernaud
contact affiliation	Universidad de La Laguna
contact email	jrfernaud@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD009915
     1. Label: PRIDE project
     2. Name: Evidence for the nuclear import of monomeric histones H3.1 & H4