PXD009902
PXD009902 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Proteomic Analysis of an Arsenic Response Locus |
| Description | Arsenic exposure is a global health problem. Millions of people encounter arsenic through contaminated drinking water, consumption, and inhalation. The arsenic response locus in budding yeast is responsible for the detoxification of arsenic and its removal from the cell. This locus constitutes a conserved pathway ranging from prokaryotes to higher eukaryotes. The goal of this study was to identify how the arsenic response locus is regulated in an arsenic dependent manner. An affinity enrichment strategy called CRISPR-Chromatin Affinity Purification with Mass Spectrometry (CRISPR-ChAP-MS) was used that provides for the proteomic characterization of a given locus. CRISPR-ChAP-MS was applied to the arsenic response locus and uncovered 40 nuclear-annotated proteins showing enrichment. Functional assays, identified the histone acetyltransferase activity of SAGA and the ATPase chromatin remodeling activity of SWI/SNF to be required for activation of the locus. Furthermore, SAGA and SWI/SNF were both found to specifically organize the chromatin structure at the arsenic response locus for activation of gene transcription. This study provides the first proteomic characterization of an arsenic response locus and key insight into the mechanism of transcriptional activation that is necessary for detoxification of arsenic from the cell. |
| HostingRepository | PRIDE |
| AnnounceDate | 2024-10-22 |
| AnnouncementXML | Submission_2024-10-22_05:03:18.213.xml |
| DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD009902 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Stephanie Byrum |
| SpeciesList | scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; |
| ModificationList | Oxidation; Acetyl; Carbamidomethyl |
| Instrument | Orbitrap Fusion |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2018-05-25 03:40:57 | ID requested | |
| 1 | 2020-05-07 02:18:11 | announced | |
| ⏵ 2 | 2024-10-22 05:03:19 | announced | 2024-10-22: Updated project metadata. |
Publication List
| 10.6019/PXD009902; |
| 10.1080/15592294.2019.1580110; |
| West KL, Byrum SD, Mackintosh SG, Edmondson RD, Taverna SD, Tackett AJ, Proteomic characterization of the arsenic response locus in S. cerevisiae. Epigenetics, 14(2):130-145(2019) [pubmed] |
Keyword List
| curator keyword: Biomedical |
| submitter keyword: Arsenic, yeast, ChAP-MS, CRISPR |
Contact List
| Alan J. Tackett | |
|---|---|
| contact affiliation | Professor of Biochemistry & Molecular Biology, Pediatrics and Pathology Full Member Arkansas Children’s Research Institute Scharlau Family Endowed Chair in Cancer Research Director COBRE Center for Translational Pediatric Research (www.archildrens.org/archildrens-COBRE) Director IDeA National Resource for Proteomics (http://idearesourceproteomics.org/) University of Arkansas for Medical Sciences |
| contact email | ajtackett@uams.edu |
| lab head | |
| Stephanie Byrum | |
| contact affiliation | UAMS |
| contact email | sbyrum@uams.edu |
| dataset submitter | |
Full Dataset Link List
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD009902 |
| PRIDE project URI |
Repository Record List
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