PXD009894

PXD009894 is an original dataset announced via ProteomeXchange.

Title	dHF proteomic following EGb treatment
Description	We have previously shown that standardized extracts of Ginkgo biloba (EGb) modulate fear memory formation, which is associated with CREB-1 (mRNA and protein) upregulation in the dorsal hippocampal formation (dHF), in a dose-dependent manner. Here, we employed proteomic analysis to investigate EGb effects on different protein expression patterns in the dHF, which might be involved in the regulation of CREB activity and the synaptic plasticity required for long-term memory (LTM) formation. Adult male Wistar rats were randomly assigned to four groups (n=6/group) and were submitted to conditioned lick suppression 30 min after vehicle (12% Tween 80) or EGb (0.25; 0.50 and 1.00 g.kg-1) administration (v.o). All rats underwent a retention test session 48 hours after conditioning. Twenty-four hours after the test session, the rats were euthanized via decapitation, and dHF samples were removed for proteome analysis using two-dimensional polyacrylamide gel electrophoresis, followed by peptide mass fingerprinting. In agreement with our previous data, no differences in the suppression ratios were identified among the groups (P>0.05). However, our results showed, for the first time, that 32 proteins were affected in the dHF following treatment with 0.25, 0.50 and 1.00 g.kg-1 doses of EGb, which upregulated 7, 19 and 5 proteins, respectively. Additionally, EGb downregulated 2 proteins at each dose. These proteins are correlated with remodelling of the cytoskeleton; the stability, size and shape of dendritic spines; myelin sheath formation; and composition proteins of structures found in the membrane of the somatodendritic and axonal compartments. Our findings suggested that EGb modulates conditioned suppression LTM through differential protein expression profiles, which may be a target for cognitive enhancers and for the prevention or treatment of neurocognitive impairments.
HostingRepository	PRIDE
AnnounceDate	2019-01-07
AnnouncementXML	Submission_2019-02-11_04:28:52.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Renan Gaiardo
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	No PTMs are included in the dataset
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2018-05-25 01:27:14	ID requested
1	2019-01-07 03:26:21	announced
⏵ 2	2019-02-11 04:28:53	announced	Updated publication reference for PubMed record(s): 30666208.

Gaiardo RB, Abreu TF, Tashima AK, Telles MM, Cerutti SM, . Front Pharmacol, 9():1533(2018) [pubmed]

curator keyword: Biomedical

submitter keyword: dHF, conditioned lick supression, Ginkgo biloba

Suzete Maria Cerutti
contact affiliation	Federal University of Sao Paulo Department of Biological Science Cellular and Behavioural Pharmacology Laboratory
contact email	smcerutti@gmail.com
lab head
Renan Gaiardo
contact affiliation	Universidade Federal de São Paulo
contact email	gaiardorb@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD009894
     1. Label: PRIDE project
     2. Name: dHF proteomic following EGb treatment