PXD009876

PXD009876 is an original dataset announced via ProteomeXchange.

Title	Proteome analysis indicates participation of the dorsal hippocampal formation in conditioned lick suppression
Description	Memory formation is an essential function for survival and cognitive flexibility that involves both synaptic and systems consolidation. The latter is an intricate process requiring integrated signalling inputs into complex neural networks over time. The purpose of the present study was to identify proteins and pathways affected by memory formation induced by the conditioned lick suppression paradigm, as well as to investigate the role of the dHF in the transfer of memory to a more permanent mode in other brain areas. To evaluate the acquisition of fear memory, a protocol of conditioned suppression of an ongoing behaviour was conducted for either 8 or 46 days. Two or 40 days after conditioning, the animals were subjected to a retention test and were then euthanized after 24 hours for dHF harvesting. There were 1,165 proteins identified and 265 quantified. The analysis of the fear memory performed on post-conditioning day 2 showed an upregulation of 5 proteins and a downregulation of 11 proteins. Additionally, an analysis on post-conditioning day 40 showed that 8 proteins were upregulated, and 32 proteins were downregulated. Integrated pathway analysis of the proteomics data indicated changes in the myelin sheath, neuron generation and differentiation, regulation of neurogenesis and synaptic vesicle transport, axonal development and the growth cone. Altogether, the current findings provide further support for the role of the dHF in conditioned lick suppression memory and novel insights into the molecular changes that are correlated with the transfer of memory from the dHF to other cortical areas, which may be a target for cognitive enhancers.
HostingRepository	PRIDE
AnnounceDate	2024-02-01
AnnouncementXML	Submission_2024-02-01_12:20:59.960.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Renan Gaiardo
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	No PTMs are included in the dataset
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2018-05-24 02:29:44	ID requested
⏵ 1	2024-02-01 12:21:00	announced

10.1037/BNE0000563;

curator keyword: Biological

submitter keyword: proteomics, memory, conditioned fear,LC-MSMS

Suzete Maria Cerutti
contact affiliation	Federal University of sao Paulo Department of Biological Science Cellular and Behavioural Pharmacology Laboratory
contact email	smcerutti@gmail.com
lab head
Renan Gaiardo
contact affiliation	Universidade Federal de São Paulo
contact email	gaiardorb@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/02/PXD009876

PRIDE project URI

• PRIDE
  1. PXD009876
     1. Label: PRIDE project
     2. Name: Proteome analysis indicates participation of the dorsal hippocampal formation in conditioned lick suppression