PXD009875

PXD009875 is an original dataset announced via ProteomeXchange.

Title	Optimizing chromatographic conditions for orbitrap-based MS platforms
Description	We compared the performance of multidimensional protein identification (MudPIT) on Velos Pro Orbitrap (VPO) and Velos Orbitrap Elite (VOE) mass spectrometers to single dimension reversed phase (RP) chromatography on a Q Exactive Plus (QE+) and an Orbitrap Fusion™ Lumos™ (OFL). Using a digested HeLa cell protein extract, we carried out 16 different chromatography conditions on four different instrumentation platforms with three replicates of each condition, for a total of 48 proteomics analyses. We first compared selected chromatography conditions of the QE+ and OFL by varying column lengths, inner diameters, and C18-RP particle sizes. We next selected one chromatography condition on each system and varied the effective RP gradients lengths
HostingRepository	PRIDE
AnnounceDate	2019-03-04
AnnouncementXML	Submission_2019-03-26_09:47:34.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD009875
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Laurence Florens
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue
Instrument	Orbitrap Fusion Lumos; LTQ Orbitrap Velos; Q Exactive; LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2018-05-24 01:05:30	ID requested
1	2019-03-04 02:30:59	announced
⏵ 2	2019-03-26 09:47:36	announced	Updated publication reference for PubMed record(s): 30884231.

Zhang Y, Wen Z, Washburn MP, Florens L, Evaluating Chromatographic Approaches for the Quantitative Analysis of a Human Proteome on Orbitrap-Based Mass Spectrometry Systems. J Proteome Res, 18(4):1857-1869(2019) [pubmed]

curator keyword: Technical

submitter keyword: Orbitrap, Q Exactive, Fusion Lumos, Quantitative Proteomics, Human, Chromatography, Pearson Product-Moment Correlation Coefficient, Reproducibility, Multidimensional Protein Identification Technology, distributed Normalized Spectral Abundance Factor, Data-Dependent Acquisition

Laurence Florens
contact affiliation	Stowers Institute for Medical Research
contact email	laf@stowers.org
lab head
Laurence Florens
contact affiliation	Proteomics
contact email	laf@stowers.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/03/PXD009875

PRIDE project URI

• PRIDE
  1. PXD009875
     1. Label: PRIDE project
     2. Name: Optimizing chromatographic conditions for orbitrap-based MS platforms