PXD009871

PXD009871 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Structural Analysis of Hippocampal Kinase Signal Transduction
Description	Kinases are a major clinical target for human diseases. Identifying the proteins that interact with kinases in vivo will provide information on unreported substrates and will potentially lead to more specific methods for therapeutic kinase regulation. Here, endogenous immunoprecipitations of evolutionally distinct kinases (i.e. Akt, ERK2, and CAMK2) from rodent hippocampi were analyzed by mass spectrometry to generate three highly confident kinase protein-protein interaction networks. Proteins of similar function were identified in the networks, suggesting a universal model for kinase signaling complexes. Structural connections were observed between kinases with reported symbiotic relationships. The kinase networks were significantly enriched in genes associated with specific neurodevelopmental disorders providing novel structural connections between these disease-associated genes. To demonstrate a functional relationship between the kinases and its network, pharmacological manipulation of Akt in hippocampal slices was shown to regulates the activity of potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel(HCN1), which was identified in the Akt network. Overall, the kinase protein-protein interaction networks provide molecular insight of the spatial complexity of in vivo kinase signal transduction which is required to achieve the therapeutic potential of kinase manipulation in the brain.
HostingRepository	MassIVE
AnnounceDate	2018-08-02
AnnouncementXML	Submission_2018-08-02_12:41:25.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Daniel McClatchy
SpeciesList	scientific name: Rattus norvegicus; common name: Norway rat; NCBI TaxID: 10116;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2018-05-23 13:34:16	ID requested
⏵ 1	2018-08-02 12:41:26	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: Protein-Protein Interactions, Hippocampus, Kinases

submitter keyword: Protein-Protein Interactions, Hippocampus, Kinases

Contact List

John R. Yates III
contact affiliation	The Scripps Research Institute
contact email	jyates@scripps.edu
lab head
Daniel McClatchy
contact affiliation	TSRI
contact email	dmcclat@scripps.edu
dataset submitter

Full Dataset Link List

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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000082390