PXD009867

PXD009867 is an original dataset announced via ProteomeXchange.

Title	Gastric Bypass and Sleeve Gastrectomy induce a new gastric mucosa phenotype with increased number of Glucagon-Like Peptide-1 expressing cells
Description	The Glucagon-Like Peptide-1 (GLP-1) is a proglucagon-derived peptide with regulatory effects on many tissues, including the pancreas, stomach, liver, brain and heart. The rapid inactivation of intestinally secreted GLP-1 by DPP-4 enzyme raises the question of its production in proximity of its targets. Here we show some epithelial cells producing GLP-1 in the stomach of rats and humans. These cells respond specifically to intragastric load of glucose by increasing GLP-1 levels in the portal vein, in vivo in the rat. GLP-1 is known as an incretin, it decreases blood glucose levels after a meal by increasing insulin secretion in response to glucose. The increased GLP-1 secretion in obese individuals who have undergone bariatric surgery is considered as a keystone in the glycemic improvement observed in those patients. Here we show that obese rats that underwent RYGB or VSG exhibit a new gastric mucosa phenotype with expansion and hyperplasia of the mucus neck cells, and increased density of gastric GLP-1 expressing cells compared to sham animals. These findings highlight a potential role of stomach-derived GLP-1 in the outcomes of bariatric surgeries and raise the question of its role in physiology and metabolism.
HostingRepository	PRIDE
AnnounceDate	2020-12-04
AnnouncementXML	Submission_2020-12-04_05:25:43.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thibaut LEGER
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	phosphorylated residue; monohydroxylated residue; acetylated residue
Instrument	Orbitrap Fusion ETD

Revision	Datetime	Status	ChangeLog Entry
0	2018-05-23 08:04:38	ID requested
⏵ 1	2020-12-04 05:25:43	announced

Dataset with its publication pending

curator keyword: Biological, Biomedical

submitter keyword: Glp1, glucagon, rattus, gastric bypass, gastrectomy

André Bado
contact affiliation	Equipe B.A.D.O (Bariatric surgery Alimentary Diseases and Obesity) Inserm UMR 1149, Centre de Recherche sur l'Inflammation Paris Montmartre UFR de Médecine Paris Diderot, 16 rue Henri Huchard, 75890 Paris Cedex 18, France Tel. (33.1) 57 27 74 60
contact email	andre.bado@inserm.fr
lab head
Thibaut LEGER
contact affiliation	Institut Jacques Monod
contact email	thibaut.leger@ijm.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/12/PXD009867

PRIDE project URI

• PRIDE
  1. PXD009867
     1. Label: PRIDE project
     2. Name: Gastric Bypass and Sleeve Gastrectomy induce a new gastric mucosa phenotype with increased number of Glucagon-Like Peptide-1 expressing cells