PXD009860

PXD009860 is an original dataset announced via ProteomeXchange.

Title	Comparative Two-Dimensional Polyacrylamide Gel Electrophoresis (2D-PAGE) of Human Milk to Identify Dysregulated Proteins in Breast Cancer
Description	Breast Cancer (BC) remains a major cause of mortality, and early detection is considered important for reducing BC-associated deaths. Early detection of BC is challenging in young women, due to the limitations of mammography on the dense breast tissue of young women. We recently reported results of a pilot study, using one-dimensional polyacrylamide gel electrophoresis (1D-PAGE)-based mass spectrometry (MS) to investigate differences in milk proteins from women with and without BC. Here, we applied two-dimensional polyacrylamide gel electrophoresis (2D-PAGE)-based MS to compare the protein pattern in milk from the breasts of a single woman who was diagnosed with BC in one breast 24 months after donating her mil. Statistically different gel spots were picked for protein digestion followed by nanoLC-MS/MS analysis. The upregulated proteins in BC versus control are alpha-amylase, gelsolin isoform a precursor, alpha-2-glycoprotein 1 zinc isoform CRA_b partial, apoptosis-inducing factor 2 and vitronectin. Several proteins were downregulated in the milk of the breast later diagnosed with cancer as compared to the milk from the healthy breast, including different isoforms of albumin, cholesterol esterase, different isoforms of lactoferrin, different proteins from the casein family and different isoforms of lysozyme. Results warrant further studies to determine the usefulness of these milk proteins for assessing risk and detecting occult disease.
HostingRepository	PRIDE
AnnounceDate	2018-06-25
AnnouncementXML	Submission_2018-06-25_05:58:04.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Roshanak Aslebagh
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue
Instrument	Q-Tof ultima

Revision	Datetime	Status	ChangeLog Entry
0	2018-05-22 08:52:46	ID requested
⏵ 1	2018-06-25 05:58:05	announced

Aslebagh R, Channaveerappa D, Arcaro KF, Darie CC, Comparative two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) of human milk to identify dysregulated proteins in breast cancer. Electrophoresis, ():(2018) [pubmed]

ProteomeXchange project tag: Human Proteome Project

curator keyword: Biomedical

submitter keyword: Breast cancer, Breast milk, Biomarkers, 2D-PAGE, Proteomics, Mass spectrometry

Costel C. Darie
contact affiliation	Clarkson University
contact email	cdarie@clarkson.edu
lab head
Roshanak Aslebagh
contact affiliation	Clarkson University
contact email	aslebar@clarkson.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD009860

PRIDE project URI

• PRIDE
  1. PXD009860
     1. Label: PRIDE project
     2. Name: Comparative Two-Dimensional Polyacrylamide Gel Electrophoresis (2D-PAGE) of Human Milk to Identify Dysregulated Proteins in Breast Cancer