PXD009858

PXD009858 is an original dataset announced via ProteomeXchange.

Title	Embryogenic competence acquisition in sugarcane callus is associated with differential H+ pump abundance and activity
Description	Somatic embryogenesis is an important biological process in several plant species, including sugarcane. Proteomics approaches have shown that H+ pumps are differentially regulated during somatic embryogenesis; however, the relationship between H+ flux and embryogenic competence is still unclear. This work aimed to elucidate the association between extracellular H+ flux and somatic embryo maturation in sugarcane. We performed a microsomal proteomics analysis and analyzed changes in extracellular H+ flux and H+ pump (P-H+-ATPase, V-H+-ATPase and H+-PPase) activity in embryogenic and non-embryogenic callus. A total of 657 proteins were identified, 16 of which were H+ pumps. We observed that P-H+-ATPase and H+-PPase were more abundant in embryogenic callus. Compared with non-embryogenic callus, embryogenic callus showed high H+ influx, especially at maturation day 14 as well as higher H+ pump activity, mainly P-H+-ATPase and H+-PPase activity. The H+-PPase appears to be the major H+ pump in embryogenic callus during somatic embryo formation, functioning in both vacuole acidification and PPi homeostasis. These results provide evidence for an association between higher H+ pump protein abundance and, consequently, higher H+ flux and embryogenic competence acquisition in the callus of sugarcane.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:13:23.623.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Vanildo Silveira
SpeciesList	scientific name: Saccharum officinarum; NCBI TaxID: 4547;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2018-05-22 07:24:49	ID requested
1	2018-06-26 08:13:13	announced
⏵ 2	2024-10-22 04:13:24	announced	2024-10-22: Updated project metadata.

10.1021/acs.jproteome.8b00213;

Passamani LZ, Bertolazi AA, Ramos AC, Santa-Catarina C, Thelen JJ, Silveira V, -Pump Abundance and Activity. J Proteome Res, 17(8):2767-2779(2018) [pubmed]

curator keyword: Biological

submitter keyword: somatic embryogenesis

ion-selective vibrating probe

Saccharum ssp.

H+ pumps

microsomal proteomics

Vanildo Silveira
contact affiliation	Laboratório de Biotecnologia, Centro de Biociências e Biotecnologia (CBB), Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Av. Alberto Lamego, Campos dos Goytacazes, RJ, Brazil. Unidade de Biologia Integrativa, Setor de Genômica e Proteômica, UENF, Campos dos Goytacazes, RJ, Brazil.
contact email	vanildo@uenf.br
lab head
Vanildo Silveira
contact affiliation	UNIVERSIDADE ESTADUAL DO NORTE FLUMINENSE
contact email	vanildo@uenf.br
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD009858
     1. Label: PRIDE project
     2. Name: Embryogenic competence acquisition in sugarcane callus is associated with differential H+ pump abundance and activity