PXD009855

PXD009855 is an original dataset announced via ProteomeXchange.

Title	MTA proteins act redundantly within the NuRD complex to ensure fidelity of ES cell lineage commitment
Description	Multiprotein chromatin remodelling complexes show remarkable conservation of function amongst metazoans, even though each component present in invertebrates are often present as multiple paralogous proteins in vertebrate complexes. In some cases these paralogues have been shown to specify distinct biochemical and/or functional activities in vertebrate cells. Here we set out to define the biochemical and functional diversity encoded by one such group of proteins within the mammalian Nucleosome Remodelling and Deaceylation (NuRD) complex: Mta1, Mta2 and Mta3. We find that, in contrast to what has been described in somatic cells, MTA proteins are not mutually exclusive within ES cell NuRD and, despite subtle differences in chromatin binding and biochemical interactions, serve largely redundant functions. Nevertheless, ES cells lacking all three MTA proteins represent a complete NuRD null and are viable, allowing us to identify a previously undetected function for NuRD in maintaining differentiation trajectory during early stages of lineage commitment.
HostingRepository	PRIDE
AnnounceDate	2019-03-26
AnnouncementXML	Submission_2019-03-26_09:47:07.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Susan Kloet
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-05-22 01:41:49	ID requested
⏵ 1	2019-03-26 09:47:08	announced

Dataset with its publication pending

curator keyword: Biological

submitter keyword: LC-MS/MS, mouse embryonic stem cell, AP-MS/MS, NuRD complex

Michiel Vermeulen
contact affiliation	Department of Molecular Biology, Radboud Institute for Molecular Life Sciences, Radboud University, Nijmegen, The Netherlands
contact email	michiel.vermeulen@science.ru.nl
lab head
Susan Kloet
contact affiliation	Radboud University
contact email	S.L.Kloet@lumc.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/03/PXD009855

PRIDE project URI

• PRIDE
  1. PXD009855
     1. Label: PRIDE project
     2. Name: MTA proteins act redundantly within the NuRD complex to ensure fidelity of ES cell lineage commitment