PXD009845 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | MiR-135a-5p is critical for exercise-induced proliferation of adult neurogenesis |
Description | Physical exercise stimulates adult hippocampal neurogenesis in mammals, and is considered a relevant strategy for preventing age-related cognitive decline in aging humans. However, its mechanism is controversial. Here, by investigating microRNAs (miRNAs) and their downstream pathways, we uncover that downregulation of miR-135a-5p mediates exercise-induced proliferation of adult NPCs in adult neurogenesis in the mouse hippocampus, likely by activation of phosphatidylinositol (IP3) signaling. Specifically, while overexpression of miR-135 prevents exercise-induced proliferation in the adult mouse hippocampus in vivo and in NPCs in vitro, its inhibition activates NPCs proliferation in resting and aged mice. Label free proteomics and bioinformatics analysis identifies 11 potential targets of miR-135 in NPCs, several of them involved in phosphatidylinositol signaling. Thus, miR-135a is key in mediating exercise-induced adult neurogenesis and opens intriguing perspectives toward the therapeutic exploitation of miR-135 to delay or prevent pathological brain ageing.Physical exercise stimulates adult hippocampal neurogenesis in mammals, and is considered a relevant strategy for preventing age-related cognitive decline in aging humans. However, its mechanism is controversial. Here, by investigating microRNAs (miRNAs) and their downstream pathways, we uncover that downregulation of miR-135a-5p mediates exercise-induced proliferation of adult NPCs in adult neurogenesis in the mouse hippocampus, likely by activation of phosphatidylinositol (IP3) signaling. Specifically, while overexpression of miR-135 prevents exercise-induced proliferation in the adult mouse hippocampus in vivo and in NPCs in vitro, its inhibition activates NPCs proliferation in resting and aged mice. Label free proteomics and bioinformatics analysis identifies 11 potential targets of miR-135 in NPCs, several of them involved in phosphatidylinositol signaling. Thus, miR-135a is key in mediating exercise-induced adult neurogenesis and opens intriguing perspectives toward the therapeutic exploitation of miR-135 to delay or prevent pathological brain ageing. |
HostingRepository | PRIDE |
AnnounceDate | 2019-11-12 |
AnnouncementXML | Submission_2019-11-12_11:19:39.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Andrea Armirotti |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | iodoacetamide derivatized residue |
Instrument | TripleTOF 5600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-05-21 01:46:54 | ID requested | |
⏵ 1 | 2019-11-12 11:19:40 | announced | |
Publication List
Pons-Espinal M, Gasperini C, Marzi MJ, Braccia C, Armirotti A, P, รถ, tzsch A, Walker TL, Fabel K, Nicassio F, Kempermann G, De Pietri Tonelli D, MiR-135a-5p Is Critical for Exercise-Induced Adult Neurogenesis. Stem Cell Reports, 12(6):1298-1312(2019) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: miRNA, Adult Neurogenesis, SWATH |
Contact List
Andrea Armirotti |
contact affiliation | Analytical Chemistry Lab, Fondazione Istituto Italiano di Tecnologia |
contact email | andrea.armirotti@iit.it |
lab head | |
Andrea Armirotti |
contact affiliation | Analytical Chemistry Facility, Fondazione Istituto Italiano di Tecnologia |
contact email | andrea.armirotti@iit.it |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD009845
- Label: PRIDE project
- Name: MiR-135a-5p is critical for exercise-induced proliferation of adult neurogenesis