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PXD009845

PXD009845 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMiR-135a-5p is critical for exercise-induced proliferation of adult neurogenesis
DescriptionPhysical exercise stimulates adult hippocampal neurogenesis in mammals, and is considered a relevant strategy for preventing age-related cognitive decline in aging humans. However, its mechanism is controversial. Here, by investigating microRNAs (miRNAs) and their downstream pathways, we uncover that downregulation of miR-135a-5p mediates exercise-induced proliferation of adult NPCs in adult neurogenesis in the mouse hippocampus, likely by activation of phosphatidylinositol (IP3) signaling. Specifically, while overexpression of miR-135 prevents exercise-induced proliferation in the adult mouse hippocampus in vivo and in NPCs in vitro, its inhibition activates NPCs proliferation in resting and aged mice. Label free proteomics and bioinformatics analysis identifies 11 potential targets of miR-135 in NPCs, several of them involved in phosphatidylinositol signaling. Thus, miR-135a is key in mediating exercise-induced adult neurogenesis and opens intriguing perspectives toward the therapeutic exploitation of miR-135 to delay or prevent pathological brain ageing.Physical exercise stimulates adult hippocampal neurogenesis in mammals, and is considered a relevant strategy for preventing age-related cognitive decline in aging humans. However, its mechanism is controversial. Here, by investigating microRNAs (miRNAs) and their downstream pathways, we uncover that downregulation of miR-135a-5p mediates exercise-induced proliferation of adult NPCs in adult neurogenesis in the mouse hippocampus, likely by activation of phosphatidylinositol (IP3) signaling. Specifically, while overexpression of miR-135 prevents exercise-induced proliferation in the adult mouse hippocampus in vivo and in NPCs in vitro, its inhibition activates NPCs proliferation in resting and aged mice. Label free proteomics and bioinformatics analysis identifies 11 potential targets of miR-135 in NPCs, several of them involved in phosphatidylinositol signaling. Thus, miR-135a is key in mediating exercise-induced adult neurogenesis and opens intriguing perspectives toward the therapeutic exploitation of miR-135 to delay or prevent pathological brain ageing.
HostingRepositoryPRIDE
AnnounceDate2019-11-12
AnnouncementXMLSubmission_2019-11-12_11:19:39.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAndrea Armirotti
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListiodoacetamide derivatized residue
InstrumentTripleTOF 5600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-05-21 01:46:54ID requested
12019-11-12 11:19:40announced
Publication List
Pons-Espinal M, Gasperini C, Marzi MJ, Braccia C, Armirotti A, P, รถ, tzsch A, Walker TL, Fabel K, Nicassio F, Kempermann G, De Pietri Tonelli D, MiR-135a-5p Is Critical for Exercise-Induced Adult Neurogenesis. Stem Cell Reports, 12(6):1298-1312(2019) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: miRNA, Adult Neurogenesis, SWATH
Contact List
Andrea Armirotti
contact affiliationAnalytical Chemistry Lab, Fondazione Istituto Italiano di Tecnologia
contact emailandrea.armirotti@iit.it
lab head
Andrea Armirotti
contact affiliationAnalytical Chemistry Facility, Fondazione Istituto Italiano di Tecnologia
contact emailandrea.armirotti@iit.it
dataset submitter
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Dataset FTP location
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