PXD009838

PXD009838 is an original dataset announced via ProteomeXchange.

Title	Stable isotope dynamic labelling of secretomes: Strataclean Linearity C4PR_LIV
Description	We improve on currently-available resources by describing a mass spectrometry (MS)-based strategy using stable isotope dynamic labelling of secretomes (SIDLS) that discriminates between authentic secretory proteins and intracellular proteins within the secretome of cultured cells. By monitoring the rate of incorporation of labelled amino acids into newly synthesised proteins as they appear in the media, we can differentiate those proteins that have been destined for secretion, and exhibit rapid labelling, from those with low rates of labelling or low turnover relative to the growth rate of the cells which is a feature of intracellular proteins. Part of the wet lab protocol for our analysis of secretomes, is the use of the resin, Strataclean (Agilent). In this Supplementary dataset to our main paper (also in PRIDE/ProteomeXchange), we characterise the linearity of capture of secreted proteins using Stracalean resin, by label-free quantitative mass spectrometry. We mix cell-coditioned culture media with 'virgin' media in different ratios and analayse the capture capacity of equal quantitites of StrataClean reagent.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:13:13.243.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dean Hammond
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-05-18 09:37:24	ID requested
1	2018-08-20 03:14:23	announced
⏵ 2	2024-10-22 04:13:14	announced	2024-10-22: Updated project metadata.

10.1074/mcp.TIR117.000516;

Hammond DE, Kumar JD, Raymond L, Simpson DM, Beynon RJ, Dockray GJ, Varro A, Stable Isotope Dynamic Labeling of Secretomes (SIDLS) Identifies Authentic Secretory Proteins Released by Cancer and Stromal Cells. Mol Cell Proteomics, 17(9):1837-1849(2018) [pubmed]

curator keyword: Technical, Biomedical

submitter keyword: Human, Secretome, Oesophageal cancer

Dean Hammond
contact affiliation	Dept. of Cellular & Molecular Physiology Institute of Translational Medicine University of Liverpool Crown Street Liverpool L69 3BX
contact email	d.e.hammond@liverpool.ac.uk
lab head
Dean Hammond
contact affiliation	University of Liverpool
contact email	d.e.hammond@liv.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/10/PXD009838

PRIDE project URI

• PRIDE
  1. PXD009838
     1. Label: PRIDE project
     2. Name: Stable isotope dynamic labelling of secretomes: Strataclean Linearity C4PR_LIV